The total gypenoside was separated and purified by silica gel column. Chloroform and methanol were used as mobile phase in gradient elution, the sample was determined by TLC. 7.87 g total saponin was obtained from 5000 g Gynostemma pentaphyllum by the process of extracted with alcohol, defatted with ligarine, leached with normal butanol, desugared with AB-8 macro porous resin, decolored with D-296 anion ion exchange resin. Three kinds of gypenoside monomer were obtained from 5 g total gypenoside by the proportion in 9.5∶0.5 and 9∶1 chloroform and methanol as mobile phase. The yields were 2.6％, 3.0％, 5.2％ and the purity was up to 88. 73％, 93.11％,78.54％ respectively. The result indicated that the silica gel column is a good separation function to gypenoside.%采用硅胶柱层析法分离绞股蓝总皂苷,以氯仿-甲醇混合液为流动相进行梯度洗脱,薄层层析法跟踪检测.5 000 g绞股蓝干草经乙醇提、石油醚脱脂、正丁醇萃取、AB-8大孔吸附树脂脱糖、D-296阴离子交换树脂脱色后得到总皂苷7.87 g.5 g绞股蓝总皂苷进行硅胶柱层析分离,在体积比为9.5:0.5和9:1的氯仿-甲醇洗脱液洗脱下得到3种较纯的绞股蓝皂苷的单体组分,得率分别为2.6%、3.0%和5.2%.经高效液相色谱检测其纯度,组分A、B、C纯度分别为88.73%、93.11%和78.54%.3种皂苷较粗品纯度得到很大提高,且洗脱液比例和体积的确定为大量制备这3种绞股蓝单体皂苷提供了理论依据.
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