全反式维甲酸对肺气肿大鼠氧化应激的影响

摘要

Objective To observe the effect of all-trans-retinoic acid (ATRA) on the oxidatie stress influence in emphysema model rats induced by pig trypsin(PPE). Methods 60 rats were randomized into control (N) and model group (E) ,cottonseed oil group (C), low doses of ATRA treatment group (L) ,middle dose ATRA treatment group (M) ,high-dose ATRA treatment group (H) every 10 for research object,with large,medium and small doses of ATRA and cottonseed oil injection rats intra-abdominal treatment. 30 days later,measuring rats lung volume, the alveolar morphology,lung tissue SOD and MDA content. Results E, C, L, M, H rats are significantly greater than N group in lung volume, alveolar morphological and lung tissue oxidative Stress ( P ＜ 0. 05 ). Lung tissue SOD of L group were significantly higher than E group ( P ＜ 0. 05 ); Lung tissue MDA content of L group were decreased than E group significantly ( P ＜ 0. 05 ). Conclusion Emphysema rat models with low doses ATRA therapy on alveolar have well repair function, it can improve lung tissue SOD, reduce lung tissue MDA content significantly ,and rise antioxidant protection function of lung tissue.%目的 观察全反式维甲酸(ATRA)对猪胰蛋白酶(PPE)致大鼠肺气肿模型的氧化应激的影响.方法 选60只Wistar大鼠随机分为对照组(N)和模型组(E),棉籽油组(C),小剂量ATRA治疗组(L),中剂量ATRA治疗组(M),大剂量ATRA治疗组(H)各10只为研究对象,用大、中、小剂量ATRA和棉籽油注入大鼠腹腔内进行治疗.30天后处死解剖大鼠,测量大鼠肺体积,肺泡形态学,肺组织SOD活力和MDA含量.结果 E、C、L、M、H组大鼠肺体积,肺泡形态学,氧化应激水平均明显大于N组(P<0.05);L组肺组织SOD活力较E组显著增高;L、M组肺组织MDA含量较E组显著降低(P<0.05).结论 肺气肿模型大鼠用低剂量ATRA治疗对肺泡有较好修复作用,可提高肺组织SOD活力,降低肺组织MDA含量,可以起到抗氧化保护肺组织作用.