间充质干细胞对内毒素诱导急性肺损伤大鼠NF-κB的影响

摘要

Objective To explore the junction of NF-kB in the pathogenesis of endotoxin-induction acute lung injury and the treatment effect of mesenchymal stem cells. Methods 90 rats were randomly and evenly divided into the following 5 groups: Croup A as the normal group was given the tail vein injection of equivalent normal saline; Croup B as the lipopolysaccharide group ( LPS ) was given 5 mg/kg LPS through tail vein injection; Croup C as the group of high-dose stem cells ( BMSCH group ) was given 5 mg/kg + BMSC 2 x lOVml 0. 5 ml LPS through tail vein injection; Croup D as the group of low - dose stem cells ( BMSCL group ) was given LPS 5 mg/kg + BMSC 1 ×10Vml 0. 5 ml; and the last group was the control group of stem cells ( BMSC group ), which was given 2 ?106/ml 0. 5 ml bone mesenchymal stem cells. At the point of 6 horns, 24 hours and 72 horns alter molding, every 6 rats were killed and their lung tissues were frozen by liquid nitrogen at - 70t. Then the expressions of TNF-a , IL-1 p , and NF-kB were detected. Results The expressions of TNF-a and IL-1 p and NF-kB in the different groups showed statistical significance. At the same time point, the expression in Croup B was higher- than that in Croup A, Croup C and Croup D ( P <0. 05 ), and the difference between Croup A and Croup E had no statistical sig nificance. Conclusion The expression of NF-kB rises in the lung tissues of endotoxin - induction acute lung injury rat injected through caudal vein, but its expression can be reduced by injecting bone mesenchymal stem cells immediately after endotoxin injection.%目的 探索NF-κB在内毒素诱导急性肺损伤的发病机理及间充质干细胞治疗机理中的作用.方法 90只SD大鼠随机分为5组:A组:正常对照组(n=18)尾静脉注射等量生理盐水;B组:内毒素组(LPS)(n=18):经尾静脉注射LPS 5 mg/kg;C组:高剂量干细胞组(BMSCH组)(n=18):经尾静脉注射LPS 5 mg/kg+BMSC 2×106/ml 0.5 ml;D组:低剂量干细胞组(BMSCL组)(n=18):LPS 5 mg/kg +BMSC 1×106/ml 0.5 ml;干细胞对照组(BMSC组)(n=18):骨髓间充质干细胞 2×106/ml 0.5 ml.分别于造模后6、24、72 h,处死动物收取标本,每个时间点6只(LPS的24 h组因1只死亡固只处死5只).取肺组织于生理盐水中迅速漂洗干净后,立即放入液氮中冷冻,后转入-70℃冰箱中保存备用,分别测定肺组织TNF-α、IL-1β及肺组织细胞核内的NF-κB表达.结果 TNF-ɑ、IL-1β及NF-κB不同组之间存在显著差异,各组同一时间点的比较:B组均较A组显著性升高,P＜0.05;C、D组低于B组,差异有统计学意义;E组与A组差异无统计学意义.结论 NF-κB在尾静脉注射内毒素诱导急性肺损伤大鼠的肺组织中表达升高.注射内毒素后立即经尾静脉注射小鼠骨髓间充质干细胞显著降低急性肺损伤大鼠的肺组织中的NF-κB的活性.