新甲型H1N1流感轻症病例血清免疫学特征分析

摘要

目的 探讨新甲型H1N1流感轻症病例血清免疫学特征.方法 选择新甲型H1N1流感轻症病例26例,留取外周血清分别进行MIP-1α,IFN-γ,TNF-α,IL-8检测,并与新甲型H1N1流感轻症病例25例治疗康复后作比较;同时选取健康21例外周血清做对照研究.结果 新甲型H1N1流感轻症病例外周血清中细胞因子MIP-1α,IFN-γ,IL-8分泌表达与康复组对照有差异性(t=2.16~47.15,P<0.05),而TNF-α表达无差异性(t=0.72,P>0.05);而康复组与健康组比较表达无差异性(t=0.75~1.75,P>0.05).结论 新甲型H1N1流感轻症病例细胞因子MIP-1α,IFN-γ,IL-8分泌表达增强,可能与病毒感染有关,治疗康复后与健康人表达一致.%Objective To explore the sero-immunity characteristics of mild cases with the new influenza A H1N1 virus. Methods The study selected 26 mild cases with new influenza A H1N1 , 25 post-rehabilitation cases with new influenza A H1N1 and 21 healthy people. MIP-1α, IFN-γ, IL-8 and TNF-α were detected by ELISA in peripheral serum. Results The expression of MlP-1α, IFN-γ and IL-8 showed significant difference between the mild cases group and the post-rehabilitation group ( t =2. 16 ~ 47. 15 , P < 0. 05 ), while there was no significant difference of TNF-α between the two groups ( t =0.72, P > 0. 05 ). The expression of MIP-1α, lFN-γ, IL-8 and TNF-α had no significant difference between the post-rehabilitation group and the healthy control group ( t = 0. 75 ~ 1. 75 , P > 0. 05 ). Conclusion The high expressions of MlP-1α, IFN-γ and IL-8 in the mild cases with the new influenza A H1N1 virus may be related with viral infection, but they can decrease back to normal through the therapeutic regimen.