抗胸腺细胞球蛋白与OCH对再生障碍性贫血小鼠治疗作用比较

摘要

Objective To study the efficacy and safety of natural-killer T cell ligand OCH in aplastic anemia (AA) mice model and compare it with that of antithymucyle globulin (ATG). Methods Sixty of Balb/c mice were divided into AA group, OCH group, ATG group and normal group with 15 mice each. The AA mice models were established in AA group, OCH group and ATG group. AA group was not given any treatment, OCH group was injected intraperitoneally with 100 μg/kg twice a week for two weeks from the first day after mice model established, ATG group was injected intravenously at a single dose of 60 mg/kg within 1 hour post-LN cell injection. The survival, peripheral blood examinations and bone marrow pathology were observed from the. beginning of model established to 60 days. Results Red blood cell (RBC) and hemoglobin (Hb) counts recovered to normal level compared with normal control on day 35 in OCH treated group and ATG treated group. WBC counts recovered to normal level on day 49 in OCH treated group and ATG treated group. Peripheral RBC, white blood cell and Hb recovered on day 60 in OCH and ATG treated groups and there was no significant difference compared with that of normal group. Bone marrow pathology results indicated that hematopoietic tissue recovered in OCH and ATG treated group on day 60 comparing with A A group. In these 2 groups, mice had gradual increase in hematopoietic cells and decrease in non-hematopoietic cells, such as tat cells. Conclusions OCH has the same curative effect as ATG tn improve bone marrow failure and raise the survival rate in AA mice model.%目的 研究自然杀伤T细胞配体OCH治疗免疫诱导再生障碍性贫血(再障)小鼠的有效性和安全性,并与抗胸腺细胞球蛋白(ATG)治疗进行比较.方法　60只小鼠分为再障组、OCH组、ATG组及正常组,每组各15只.再障组、OCH组、ATG组建立再生障碍性贫血模型.再障组不给予任何治疗;OCH组自造模后第1天开始腹腔注射OCH,100 μg/kg,每周2次,连续2周;ATG组白造模后1h内给药,尾静脉注射ATG60 mg/kg,单次.正常组小鼠不作任何处理.自造模开始至60 d,观察各组小鼠的生存情况、外周血常规恢复及骨髓病理改变.结果　造模后第35天,OCH组及ATG组小鼠外周血红细胞及血红蛋白恢复至正常组水平;造模后第49天OCH组及ATG组小鼠白细胞恢复至正常组水平;造模后第60天OCH组及ATG组外周血红细胞、白细胞、血红蛋白三系与正常组比较差异无统计学意义;造模后第60天OCH组与ATG组小鼠骨髓病理显示造血组织较再障组明显恢复,造血细胞数目增多,脂肪细胞等非造血细胞数目减少.结论　OCH同ATG样,可以改善免疫诱导再障小鼠的骨髓衰竭,增加其存活率.