益气养阴通络方对IgA肾病模型大鼠肾脏组织肿瘤坏死因子-α的影响

摘要

Objective To explore the effect of yiqi yangyin tongluo regime on hematuria,proteinuria and renal tissue's pathological changes in rats with IgA nephropathy (IgAN).Methods Sixty clean male SD rats were randomized into blank group,model group,huangkui group and high,medium and low-dose TCM (yiqi yangyin tongluo regime) group.After modeling for 0 week,4 weeks,8 weeks,12 weeks and 16 weeks,the levels of urine red blood cell count and 24 h urine protein were detected.Meanwhile,after feeding for 8 weeks,the pathological changes of remnant renal tissues and expression of TNF-α were detected by HE staining and immunohistochemical staining.Results After feeding for 8 weeks,compared with blank group,the level of urine red blood cell count,24h urine protein and TNF-α expression in model group,huangkui group,high,medium and low-dose TCM group were significantly higher (P ＜ 0.05).Compared with the model group,the level of urine red blood cell count,24h urine protein and TNF-α expression in huangkui group,high,medium and low-dose TCM group were significantly lower (P ＜ 0.05).The above indexes in TCM group were significantly lower than huangkui group (P ＜ 0.05).Light microscopy showed that the renal tissues were normal in blank group.In the model group,most of glomerular mesangial cells and matrix appeared moderate and severe hyperplasia,inflammatory ceils and focal fibrosis were seed in renal interstitium.Proliferation of mesangial cells and matrix were relieved in huangkui group and TCM group.Conclusion Yiqi yangyin tongluo regime can effectively ameliorate the levels of urine red blood cell count,24 h urine protein and renal pathological changes.The mechanism may be related to the decrease of urine red blood cell count and 24 h urinary protein,and inhibition of TNF-α expression.%目的 探讨益气养阴通络方对IgA肾病模型大鼠血尿、蛋白尿及对其肾脏组织病理的影响.方法 60只雄性清洁级SD大鼠,随机分为空白组、模型组、黄葵组与中药(益气养阴通络方)高、中、低剂量组,于造模前及造模后第4、8、12、16周末对各组大鼠24 h尿蛋白定量、尿红细胞计数进行检测,同时给药后8周分别通过HE染色法、免疫组化法检测各组大鼠肾组织病理变化及TNF-α表达情况.结果 与空白组比较,模型组、黄葵组、中药高、中、低剂量组大鼠给药8周后尿红细胞计数、24 h尿蛋白、TNF-α表达均不同程度增高(P＜0.05);与模型组比较,黄葵组、中药高、中、低剂量组给药8周后尿红细胞计数、24h尿蛋白、TNF-α表达显著降低(P＜0.05),且中药组给药8周上述指标均显著低于黄葵组(P＜0.05).光镜显示空白组大鼠肾组织结构正常,模型组大鼠多数肾小球系膜细胞和基质出现中、重度增生,肾间质出现炎性细胞浸润伴灶状纤维化;各治疗组肾小球系膜细胞和系膜基质增生程度均有一定程度缓解.结论 益气养阴通络方可减少IgA肾病模型大鼠血尿、蛋白尿,减轻肾脏组织病变,其作用机制可能与其降低尿红细胞数、24h尿蛋白,抑制肾脏TNF-α表达有关.