Objective To analyze and detect the diagnostic significane of the anti-Sa antibody,anti-CCP antibody,GPI antigen and rheumatoid factor in patient with RA.Methods Anti-Sa antibody,anti-CCP antibody,GPI antigen were deteceted by ELISA,RF were deteceted by immunoturbidimetric assay.Results The specificitiy and sensitivity of anti-Sa antibody,anti-CCP antibody,GPI antigen,RF were 95.69％ and 59.79％,98.28％ and 90.72％,76.14％ and 78.35％,67.24％ and 91.75％,The specificitiy and sensitivity of RF-IgA,RF-IgG,RF-IgM were 89.66％ and 58.76％,90.52％ and 68.04％,79.31％ and 79.38％.Specificitiy and sensitivity were 92.24％-100.00％ and 47.42％-86.60％ as two index turned out to be positive; were 99.14％-100.00％ and 46.39％-71.13％ as three index,were 100.00％ and 46.39％ as four index.The RA patients with anti-Sa were statistically different from the patients without this antibody in C-reactive protein.Conclusion Dictation of anti-Sa antibody,anti-CCP,GPI and rheumatoid factor can greatly improve the specificity for diagnosis of early-stage RA.%目的 探讨抗Sa抗体、抗环瓜氨酸肽(CCP)抗体、葡萄糖6-磷酸异构酶(GPI)抗原及类风湿因子(RF)联合检测对类风湿关节炎(RA)诊断的意义.方法 采用酶联免疫吸附试验(ELISA)检测抗Sa抗体、抗CCP抗体及RF亚型RF-IgA、RF-IgG、RF-IgM,免疫比浊法检测RF.结果 抗Sa抗体、抗CCP抗体、GPI抗原、RF对RA诊断的特异性和敏感性分别为95.69％和59.79％、98.28％和90.72％、76.14％和78.35％、67.24％和91.75％.RF-IgA、RF-IgG、RF-IgM对于RA诊断的特异性和敏感性分别为89.66％和58.76％、90.52％和68.04％、79.31％和79.38％.两项指标联合检测对RA诊断的特异性和敏感性为92.24％ ～ 100.00％和47.42％～86.60％;3项指标联合检测对RA诊断的特异性和敏感性为99.14％～100％和46.39％～71.13％;4项指标联合检测对RA诊断的特异性和敏感性为100.00％和46.39％.抗Sa抗体阳性组患者C反应蛋白水平高于阴性组(P＜0.05).结论 两项及两项以上指标联合检测可以显著提高RA诊断的特异性,有利于RA的早期诊断及治疗.
展开▼
