兰州地区丙型肝炎病毒基因变异与干扰素α应答关系的探讨

摘要

Objective To study the relationship between gene mutation of 5' noncoding region (5'NCR) of hepatitis C virus (HCV)genotype 1b in infection state and effect of interferon-alpha (IFNα) on the treatment of chronic hepatitis C.Methods Sera from 9patients were collected before, during and after IFNα therapy and sera from 40 HCV 1b patients were collected.HCV 5'NCR fragments were amplified from these samples by RT-PCR and sequenced by cutting with restriction endonuclease Mbo I and BamH I.Results There were 5 infection states in 40 patients with HCV 1b: (1) Mbo I restriction site was detected in 24 patients (24/40, 60.0％); (2)Mutation without Mbo I restriction site was detected in 5 patients (5/40, 12.5％); (3) 7 patients (7/40, 17.5％) coinfected the strain with or without of Mbo I restriction site; (4)Mutation with BamH I restriction site was detected in 2 patients(2/40, 0.5％); (5) 2 patients (2/40,0.5％) presented with two Mbo I restriction sites.Then we checked the mutation of 5'noncoding region of the sera from 9 patients with IFNα therapy.In the 5 patients with sustained virological response (SVR), 2 for HCV genotype 2a, 3 for HCV genotype 1b without Mbo I restriction site.In the 4 patietns with nonresponder, 1 for coinfection of HCV genotype 1b and 2a, 3 for HCV genotype 1b without Mbo I restriction site.Conclusion Of 40 HCV genotype 1b serum samples, 60.0％ patients presented single Mbo I restriction site, suggesting the state of infection with wild type strain.%目的 探讨兰州地区丙型肝炎病毒(HCV)Ib基因型5'非编码区(5'NCR)基因变异株的感染状态,及其与干扰素α疗效的关系.方法 应用限制性内切酶酶切分析检测40例HCV 1b型中5'-NCR基因变异及9例干扰素α治疗患者中的5'-NCR基因变异.结果 在40例HCV 1b型中存在5种感染状态:(1)有Mbo I切点24/40(60.0%);(2)无Mbo I切点变异株5/40(12.5%);(3)有Mbo I和无Mbo I切点混合感染株7/40(17.5%);(4)有BamH I切点变异株2/40(5%);(5)有Mbo I双切点变异株2/40(5%).对9例干扰素a治疗的丙型肝炎患者血清进行5'-NCR变异株检测.5例干扰素α应答病例中,2例为2a型,3例为无Mbo I切点的1b型.4例抗干扰素α病例中.1例为2a型,但在某节段存在着1b与2a混合状态,另3例为无Mbo I.结论 在丙型肝炎患者血清中存在单一的Mbo I切点的样品,24/40(60.0%)可能是HCV野生株感染的状态.