原发性胆汁性肝硬化患者外周血单个核细胞Toll样受体表达与调节性T细胞相关性分析

摘要

Objective To investigate the expression of toll like receptors (TLR) on peripheral blood mononuclear cells ( PBMC) and CD4 + CD25 + T regulatory cells in patients with primary biliary cirrhosis (PBC)- Methods We analysed TLR2,4 .9 on PBMC and CD4 + CD25 + Tregs of 52 PBC patients and 22 normal controls using flow cytometry. We compared the parameters between patients and normal con-trols. Results CD4 + CD25 + T regulatory cells in PBC patients were lower than that of normal controls [ (1.53 ±1-33) vs (4. 42 ± 1.43 ) , P <0. 0001 ] , however, TLR4 cells in PBC patients were higher than that of normal controls [ ( 36, 95 ± 3, 53 ) vs ( 32, 84 ± 8. 06 ) , P = 0, 003], Tregs and TLRS cells were negatively correlated in PBC patients (R2 -0- 115, P =0. 016) , whereas only Tregs were negatively correlated with TLR2 ,4/TLR2,4,9 cells in normal controls ( R2 = 0. 326,P = 0. 007; R2 = 0. 226, P = 0. 034). Conclusion Tregs are defective numerically in PBC patients and the expression of TLR4 cells is enhanced, especially in patients with infection. Tregs may affect nu-merically by infection, while they are defective numerically in PBC patients.%目的 探讨原发性胆汁性肝硬化(PBC)患者外周血单个核细胞(PBMC)Toll样受体2、4、9(TLR2,TLR4,TLR9)表达及PBMC中CD4+ CD25+调节性T细胞(Tregs)特点和影响因素.方法 采用流式细胞术检测PBC患者(52例)和健康对照者(22例)外周血PBMC中TLR2、4、9阳性细胞及Tregs比例,比较其在PBC患者和健康对照者中的差别并分析TLR与Tregs相关性.结果 PBC患者Tregs比例低于健康对照者[(1.53+1.33)vs(4.42±1.43),P＜0.0001],TLR4阳性细胞比例高于健康对照者[(36.95±3.53)vs(32.84±8.06),P=0.003]；PBC患者Tregs比例与TLR9阳性细胞比例呈负相关(R2 =0.115,P=0.016),健康对照者Tregs比例与TLR2,4和TLR2,4,9联合表达阳性细胞比例呈负相关(R2=0.326,P=0.007；R2 =0.226,P=0.034).结论 PBC患者外周血PBMC中Tregs比例降低,TLR4表达升高,可能受肝硬化失代偿期腹腔感染的影响.