E-钙粘蛋白和MMP-9在肾间质纤维化大鼠肾组织中的表达及其意义

摘要

目的 观察E-钙粘蛋白、基质金属蛋白酶-9(MMP-9)在肾间质纤维化(RIF)大鼠肾组织中的表达,探讨E-钙粘蛋白、MMP-9在RIF中的作用.方法 选用 SD大鼠72只.随机分为正常对照组、假手术组和模型组,制备单侧输尿管梗阻 UUO 模型.采用病理及免疫组织化学等方法 动态观察各组大鼠第1天、第3天、第7天、第14天肾脏组织学变化和E-钙粘蛋白、MMP在肾小管间质中表达情况.结果 UUO模型组大鼠肾小管间质损害明显,肾小管上皮细胞E-cadherin表达逐渐下调,于第14天达最低值.肾小管上皮细胞MMP-9表达上调,第3、7、14天与同期正常对照组、假手术组相比差异显著.肾小管上皮细胞E-钙粘蛋白表达量与肾小管间质损害程度呈负相关,MMP-9表达量与肾小管间质损害程度呈正相关.结论 E-钙粘蛋白表达下调、MMP-9表达上调、E-钙粘蛋白与MMP-9表达失衡可能是肾间质纤维化形成促进因素之一,并可能参与肾间质纤维化形成的全过程.%Objective To observe the expression of E - cadherin and matrix metalloproteinase - 9 ( MMP - 9 ) at kidney in rats with renal interstitial fibrosis ( RIF ), and explore the effects of E - cadherin and MMP - 9 in RIF. Methods seventy - two male Sprague - Dawley ( SD )rats were randomly divided into normal control group ( n = 24 ), sham - operated group ( n = 24 ) and model group ( n = 24 ). Tubulointerstitial fibrosis model was established via unilateral ureteral obstruction ( UUO ). Histological changes were observed under microscope and immunohistochemistry staining was performed to investigate the expression of E - cadherin and MMP - 9 in RIF by semiquantitative score at 1, 3, 7 and 14 days after experiment onset. Results Obvious tubulointerstitial injury was observed in UUO model. In model group the expression of E - cadherin in the tubular cells decreased and reached the minimum on day 14; the expression of MMP - 9 in the tubular cells increased significantly as compared to the normal group and sham - operated group on days 3, 7 and 14. There was negative correlation between renal interstitial injury and E -cadherin expression and positive correlation between renal interstitial injury and MMP - 9 expression. Conclusion The lower expression of E -cadherin and higher expression of MMP - 9 induced inequality between E - cadherin and MMP - 9 may promote the progress of RIF and play an important role in the whole process of RIF.