EGFR外显子19、21突变等位基因特异性PCR检测方法的建立

摘要

Objective To establish an allele - specific PCR method for detecting exon 19 and 21 mutation of EGFR. Methods Based on wild type and mutant exon 19 and 21 of EGFR, specific primers were designed. Genome DJNA of nucleated cells in whole blood from healthy people and patients with non - small cell lung cancer was extracted and subjected to EGFR exon 19 and 21 - specific PCR amplification. Results Deletion mutations in exon 19 and missense mutation in exon 21 were amplified with specific primers. Conclusion Allele - specific PCR method can be employed for detection of EGFR exon 19 and 21 mutations in human.%目的 建立一种人表皮生成因子受体(EGFR)外显子19、21突变等位基因特异性聚合酶链反应(PCR)检测方法.方法 根据EGFR外显子19、21野生型和突变型设计特异性引物,提取健康人和非小细胞肺癌患者血中有核细胞的基因组DNA作为模板,采用等位基因特异性聚合酶链式反应扩增EGFR外显子19、21突变基因.结果 特异性引物可以扩增EGFR外显子19缺失突变的2种类型和外显子21的错义突变.结论 等位基因特异性PCR法可以用于检测人EGFR外显子19、21突变.