经门静脉输注血小板对肝硬化大鼠部分肝切除术后残肝再生的影响研究

摘要

Objectiye This study aims to explore the influence of platelet administration via portal vein on liver regeneration of cirrhotic rats with 30% hepatectomy. Methods In order to establish the model of liver cirrhosis,we used the method of gavage with Carbon tetrachloride oily mixture. According to different interfered factors,cirrhotic rats was divided into the following three groups:platelet injection via the portal vein after 30% hepatectomy group(group 1,n = 18);saline injection via portal vein after 30% hepatectomy group(group 2,n = 18);the only 30%hepatectomy group(group 3,n = 18);another 16 normal SD rats after 30% hepatectomy treated as a normal control group(group 4,n = 16). The liver regeneration were calculated through the calculation of the residual liver weight / body weight ratio and the check of ki67 by immunohisto-chemical staining mothed in postoperative 12 h,24 h and 48 h. Results The residual liver weight / body weight ratio in 48 hours after hepatecto-my in group 1 and group 4 is much more higher than group 2 and 3 with statistical significance( P < 0. 05);liver function in each group shows no statistically significant in 12 h,24 h and 48 h after surgery( P > 0. 05). HE staining of liver cirrhosis tissues showed more proliferation in con-nective tissues,obvious pseudolobules,vacuoles deformation in hepatocellular cells etc. Immunohistochemical results showed that Ki - 67 positive stained cells increased over time. Compared with group 2 and group 3,the proportion of proliferating stained cells in group 1 increased significantly with statistical significance( P < 0. 05). Conclusion Platelet injection via portal vein can promote liver regeneration in cirrhotic rat after partial liver resection and has no significant effect on liver function.%目的：探讨经门静脉输注血小板对肝硬化大鼠行部分肝切除术后残肝再生的影响。方法采用四氯化碳油性混合液灌注，建立肝硬化模型。模型制作成功的 SD 大鼠行30%肝切除后按处理因素的不同分为以下3组：经门静脉注射血小板组（组1，n =18）；经门静脉注射生理盐水组（组2，n =18）：仅行30%肝切手术组（组3，n =18）；另取16只正常 SD 大鼠，行30%肝切作为正常对照组（组4，n =16）。术后12 h、24 h、48 h 测定大鼠血清肝功能，通过计算残肝重量与体重比和肝脏组织 Ki67免疫组化染色来观察肝再生情况。结果术后48 h 所测得的剩余肝脏与体重比值中，组1、组4 较组2、组3 高，差异具有统计学意义（ P <0.05）；在术后12 h、24 h、48 h，各组肝功能变化差异无统计学意义（ P ＞0.05）。肝硬化组别 HE 染色显示结缔组织增生明显，假小叶形成明显，部分肝细胞体积增大，空泡变形，部分肝细胞肝窦间隙增宽。免疫组化结果显示，组1 12 h、24 h、48 h 后，发现有 Ki -67阳性染色细胞，且与组2、3相比，阳性染色细胞的比率明显增加，差异具有统计学意义（ P <0.05）。结论经门静脉注射血小板能促进肝硬化大鼠部分肝切术后肝再生，且对肝功能无明显影响。