大鼠肝脏缺血-再灌注损伤时齐墩果酸对氧自由基的影响

摘要

Objective To investigate the effects of oleanolic acid (OA) on oxygen free radicals in liver ischemia-reperfusion(IR) injury in rats. Methods One hundred and twenty-eight male SD rats were randomly divided into four groups of sham operation(SH), IR, sodium carboxymethycellulose(CMC-Na), and oleanolic acid (OA). Rats I-R model was established by segmental (70%) hepatic ischemia for 60 min, followed by reperfusion for different times. The animals were sacrificed at 0, 3, 6 or 12 h after reperfusion, respectively. The activity of serum ALT, content of malondialdehyde (MDA), activity of superoxide dismutase(SOD) and content of glutathione (GSH) were measured. Results At 3 h, 6 h or 12 h after reperfusion, the activity of ALT and the content of MDA were significantly higher in group IR, CMC-Na and OA than those in group SH and the levels of SOD and GSH were lower in group IR,CMC-Na and OA than those in group SH(P<0.05). The activity of ALT and the content of MDA were significantly lower but SOD and GSH were higher in group OA than those in group IR and CMC-Na at 3 h, 6 h, or 12 after reperfusion(P<0.05). Conclusion OA can protect the liver from IR injury, which is related to the inhibition of production and release of oxygen free radicals.%目的 探讨齐墩果酸(OA)对大鼠肝脏缺血-再灌注损伤时氧自由基的影响.方法 128只雄性SD大鼠随机分为假手术组(SH组)、缺血-再灌注组(IR组)、羧甲基纤维素钠(CMC-Na组)和OA组.建立70%肝脏缺血-再灌注模型,测定肝脏缺血60 min再灌注0、3、6、12 h后血清ALT活性和肝组织丙二醛(MDA)水平、超氧化物歧化酶(SOD)活性、谷胱甘肽(GSH)水平.结果 再灌注3、6、12 h,IR组、CMC-Na组、OA组血清ALT活性、肝组织MDA水平分别显著高于SH组(P<0.05),肝组织SOD活性、GSH水平分别明显低于SH组(P<0.05);OA组ALT活性、MDA水平分别较IR组和CMC-Na组明显降低(P<0.05),SOD活性和GSH水平分别较IR组和CMC-Na组显著升高(P<0.05).结论 OA对肝脏缺血-再灌注损伤具有一定保护作用,其抗肝脏缺血-再灌注损伤作用与抑制自由基的生成和释放有关.