A new amperometric immunosensor for the determination of carcinoembryonic antigen (CEA) has been developed by means of immobilization carcinoembryonic antibody onto the nano-Au/poly (toluidine blue O)/MWNT-CS modified composite film. Under optimal conditions, the immunosensor displayed a high-sensitivity for the detection of CEA and had good correlation for the detection of CEA in the range of 1.0 to 80.0 ng/ml with a detection limit of 0.4 ng/ml. The proposed methodology was economical, efficient and potentially attractive for clinical immunoassays.%以固栽于玻碳电极(GC)表面的壳聚糖/多壁碳纳米管(MWNT-CS)复合物膜为基底电聚合媒介体甲苯胺蓝(PTOB),利用静电作用吸附纳米金(nano-Au),再利用纳米金良好的生物兼容性、大的比表面积固定癌胚抗体(anti-AFP),从而制得高灵敏、高稳定电流型癌胚抗原免疫传感器.多壁碳纳米管复合物膜促进了电子的传递,增大了电极的比表面积;纳米金的存在增加了抗体在电极表面的固定量,从而提高了免疫电极的灵敏度.在优化的实验条件下,该免疫电极在1.0～80.0 ng/mL范围内,峰电流与CEA抗原的浓度呈良好的线性关系,检测线为0.4 ng/mL.该免疫传感器制备方法简单,灵敏度高、选择性好.
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