经皮肾交感神经射频消融术对慢性心力衰竭犬肾胺酶活性的影响

摘要

Objective To investigate the effect of renal sympathetic denervation (RSD) on the activity of renalase in dogs with chronic heart failure (CHF).Methods After induced by abdominal aorta constriction,dogs were divided into three groups according to whether they underwent double renal artery ablation:2 dogs in control group,2 dogs in sham-operated group (no renal artery ablation),and 5 dogs in RSD group (renal artery ablation).Plasma noradrenaline (NE),B-type natriuretic peptide (BNP),and renalase were determined in 5 dogs with RSD (RSD group),2 control dogs (control group),and 2 shamoperated dogs (sham-operated group).Results NE,BNP and heart rate were significantly higher and renalase was lower in CHF group than those in control group (all P ＜ 0.05).Compared to the control dogs with CHF,the levels of renalase were significantly increased in 6 weeks after RSD [(1 948.78 ±49.19) ng/ml vs (1 847.35 ±20.72)ng/ml,P =0.029],and NE [(166.30 ±7.68)pg/ml vs (181.29 ±8.57)pg/ml],and BNP [(75.10 ± 5.58)lμg/ml vs (89.79 ± 2.04) μg/ml] were decreased in 8 weeks after RSD (all P ＜ 0.05).An decreased trend of the levels of renalase was observed in 8 weeks than in 6 weeks in CHF dogs after RSD,without significant difference (P ＞ 0.05).Conclusions The activity of renalase in dogs with CHF can be affected by RSD.%目的 探讨经皮肾交感神经射频消融术(RSD)对慢性心力衰竭(心衰)犬肾胺酶活性的影响.方法 采用腹主动脉结扎的方法建立犬慢性心衰模型,根据是否行RSD术分为三组,心衰对照组(2只),心衰假手术组(2只,导管入肾动脉但不消融),RSD组(5只,行RSD术).观察RSD术后不同时间心衰犬血浆去甲肾上腺素(NE)、脑钠尿肽(BNP)及肾胺酶的差异.结果 与对照组相比,心衰犬BNP增高,心率加快,NE增加,肾胺酶活性降低(P均＜0.05).与心衰对照组相比,RSD组术后第6周肾胺酶活性增高[(1 948.78±49.19) ng/ml vs(1 847.35±20.72) ng/ml,P=0.029],术后第8周NE[(166.30±7.68) pg/ml vs (181.29±8.57)pg/ml]和BNP水平[(75.10±5.58)μg/ml vs (89.79 ±2.04) μg/ml]降低(P＜0.05).RSD组术后第8周肾胺酶活性较术后第6周呈现下降趋势,差异无统计学意义(P＞0.05).结论 短期内RSD能影响慢性心衰犬肾胺酶活性,其可能通过调节肾胺酶活性影响心功能.