Objective: To investigate the influence of nutrients on the expression of insulin receptor and its related substrate genes in the mice muscle.Methods: C57BL/6J mice were randomly divided into 4 groups: ①HF, mice on high fat diet; ② G + F, supplemented with glutamine after 3 months on high fat diet; ③Gln, high fat with glutamine;④Control,normal diet. Each group has been on the diet for 5.5 months. RT-PCR was used to determine the mRNA levels of insulin receptor (IR), insulin receptor substrate-1(IRS-1), insulin receptor substrate-2(IRS-2) and phosphatidylinositol 3-kinase(PI-3-ki- nase). Results: The results showed that the mRNA levels of IR, IRS-1, IRS-2 and PI-3 kinase of the mice on high fat diet were lowered to some degree, they were 81%,63%,59% and 45% of that of control respectively. Cnoclusion: Glutamine can prevent reduction of the mRNA level with the above-mentioned genes in fat group. The results suggest that nutrients may influence insulin sensitivity through regulation of gene expression.
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