选择(28±1)日龄、平均体重为(8.84 ±0.21)kg的健康三元(斯格×长×大)杂交断奶仔猪(公母各半)18头,研究1 mg/kg玉米赤霉烯酮(zearalenone,ZEA)污染饲粮对断奶仔猪脾脏和外周血淋巴细胞增殖率和IL-2产量的影响,同时评价改性蒙脱石的脱毒效应.将试验仔猪按照体重随机分为3个处理(公母各半),仔猪采用试验笼单独饲养.试验处理为:Contr.=基础饲粮；ZEA+CZ0=基础饲粮+1 mg/kg ZEA；ZEA+ CZ4=基础饲粮+1 mg/kg ZEA+4g/kg改性蒙脱石.预饲期7d,正式期22 d.结果表明:1)与对照组相比,饲粮添加1 mg/kg ZEA显著降低断奶仔猪脾脏相对重量、外周血和脾脏淋巴细胞增殖率以及IL-2产量(P＜0.05).2)1 mg/kg的ZEA处理饲粮添加4 g/kg改性蒙脱石能够显著改善ZEA诱导的脾脏和外周血淋巴细胞增值率以及IL-2的改变(P＜0.05).结果揭示,1 mg/kg的ZEA足以影响断奶仔猪脾脏的生长发育及其细胞免疫功能,添加4 g/kg改性蒙脱石具有显著的脱毒效应,以上结果对指导动物生产和人类健康具有重要的借鉴意义.%(Objective) A total of eighteen 28 day-old postweaning piglets (L × Y × D) (half male and half female) with an average body weight of(8.84 ± 0.21) kg were used in the study.The aims of the present study were to investigate the toxicity of zearalenone(ZEA) on lymphocyte proliferation rate and IL-2 in peripheral blood and spleen of piglets;the efficacy of modified montmorillonite (CZ) in preventing ZEA-induced adverse effects had also been evaluated.(Method)The piglets were randomly divided into 3 treatments,and fed individually in a metabolic cage for 22 days after 7-d adaptation.Treatments were:Contr.=basal diet only; ZEA + CZ0 =basal diet + 1 mg/kg ZEA ; ZEA + CZ4 =basal diet + 1 mg/kg ZEA + 4 g/kg CZ.(Result) The results showed as follows:1) Piglets fed ZEA contaminated diet without CZ showed significant decreases in relative weight of spleens,lymphocyte proliferation rate (LPR) and IL-2 in peripheral blood and spleen when compared with the control (P ＜ 0.05).2) The evident improved effect was observed when added of 4 g CZ/kg diet in 1 mg/kg ZEA contaminated diet (P ＜ 0.05).(Conclusion) The above findings indicate that ZEA at level of 1 mg/kg is sufficient to induce immunotoxicity of piglets.Addition of 4 g CZ/kg diet in 1 mg/kg ZEA contaminated diet can effectively protect against the detrimental effects of the ZEA feeding.These results may have implications for animals' production and humans' health consuming ZEA-contaminated food or feed.
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