紫外分光光度法测定奥美拉唑清洁残留物方法学验证实验

摘要

Objective To validate the accuracy of determination of cleaning residue in omeprazole by UV Spectrophotometry.Methods 0.1 mol/L NaOH solution was used as solvent. UV spectrophotometry was used and its detective wave length was 305 nm.Results It was good linear relationship in the rang of 2~16μg/mL The accuracy was 0.5%, and the precision was 0.3%. The limit of detection and limit of quantification were 0.045μg/mLand 0.14μg/mL Wiping the recovery rate was more than 70.92%.Conclusion UV Spectrophotometry is a simple and an easy way to detect the cleaning residue in omeprazole with high accuracy and good precision.%目的：验证紫外分光光度法测定奥美拉唑清洁残留物的准确性。方法以0.1 mol/L NaOH溶液为溶剂，采用紫外分光光度法，检测波长为305 nm。结果该检验方法在2～16μg/mL范围有较好线性关系，准确度与精密度分别为0.5%、0.3%，检测限与定量限分别为0.045μg/mL、0.14μg/mL。擦拭法的回收率大于70.92%。结论此方法简单易操作，准确度、精密度良好。