姜黄素预处理后大鼠急性腹膜炎腹膜肿瘤坏死因子α的检测

摘要

目的 揭示姜黄素在由表皮葡萄球菌(S.epidermidis)所致的腹膜炎中对实验动物腹膜肿瘤坏死因子α(TNF-α)表达的影响.方法 大鼠腹腔注射表皮葡萄球七前菌诱发急性腹膜炎,采用Western blot和逆转录聚合酶链反应,分别在造模后3,6,12,24和48 h各时点检测姜黄素处理组与无姜黄素处理组大鼠腹膜TNF-α蛋白和mRNA的表达情况.结果 大鼠腹膜TNF-α蛋白和mRNA的表达自感染后6～24 h出现时间依赖性升高.与无姜黄素处理组相比,姜黄素处理组实验动物腹膜TNF-α蛋白和mRNA表达降低.此外,姜黄素处理组腹膜细胞损伤较无姜黄素处理组轻.结论 姜黄素在由表皮葡萄球菌引起的急性腹膜炎中具有保护作用.%Objective To investigate the effects of curcumine treatment on the expression of TNF-α in the peritoneum of acute peritonitis rats. Methods The rats were intrapertoneally injected Staphylococcus epidemidis (S.epidermidis) for the mixlelization of acute peritonitis. After a survival time of 3,6,12,24 and 48 hours,TNF-α expression in the peritoneum was detected by Western blot and RT-PCR in the rats of curcumin treatment group and non-curcumin treatment group. Results TNF-a expression showed time-dependent increase at 6,12,24 hours after the infection in the non-curcumin treatment group. In curcumin treatment group,TNF-α expression decreased at 6,12,24 hours after infection compared to non-curcumin treatment group. Conclusion Curcumin may be a protective agent in acute peri-tonitis induced by S.epidermidis.