目的 研究14-3-3蛋白在1-细胞期小鼠受精卵有丝分裂中的作用.方法 RT-PCR技术鉴定小鼠受精卵14-3-3蛋白亚型.采用显微注射方法将14-3-3 siRNA注射入小鼠受精卵G1期,观察受精卵的卵裂率、形态学变化及MPF活性.结果 小鼠受精卵中的14-3-3蛋白亚型是14-3-3ε.小鼠受精卵注射pSUPER-14-3-3ε siRNA后,与对照组相比,卵裂率下降,有丝分裂延迟,有更多的受精卵发生形态异常,MPF活性最高值显著下降.结论 14-3-3蛋白在调节小鼠受精卵有丝分裂中发挥重要作用.%Objective To study the effects of 14-3-3 proteins in regulation of the first mitotic cell cycle in one-cell stage mouse fertilized eggs. Methods 14-3-3 isoform in the mouse fertilized eggs was identified by RT-PCR. 14-3-3 siRNA was introduced to G1 phase fertilized eggs by microinjection to study the cleavage rate, morphology and MPF activity. Results 14-3-3 ε was identified in one-cell stage of mouse fertilized eggs. Compared with the control group,the cleavage rate in pSUPER-14-3-3ε siRNA injection group was significantly decreased, mitosis was delayed and more abnormal morphology eggs were observed. Moreover, the maximal value of MPF activity was significantly decreased. Conclusion 14-3-3 proteins play critical roles in the first mitotic cell cycle in mouse fertilized eggs.
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