壬基苯酚对H9c2心肌细胞钙信号和细胞增殖的影响及其作用机制

摘要

目的：探讨壬基苯酚（NP）对H9c2心肌细胞内钙离子浓度和细胞增殖的影响以及GPR30受体的作用。方法采用细胞内钙测定方法来记录H9c2心肌细胞内钙离子浓度变化，采用MTT法来观察H9c2心肌细胞增殖程度。结果1×10-10 mol/L NP使细胞内钙离子浓度升高的幅度增加，并促进H9c2细胞增殖，而1×10-6 mol/L NP使细胞内钙离子浓度升高的幅度降低，并抑制细胞增殖。G15可以阻断1×10-10 mol/L NP对细胞内钙离子浓度和细胞增殖的促进作用，但不能阻断1×10-6 mol/L NP对细胞内钙离子和细胞增殖的抑制作用。结论 NP对H9c2细胞的快速钙信号变化和细胞增殖具有非单调的剂量依赖性作用，其机制可能是由于GPR30受体在不同浓度下参与的机制不同。%Objective To observe the effects of nonylphenol(NP)on the intracellular calcium concentration changes and cell proliferation,and the involvement of GPR30 receptor in H9c2 cell. Methods The intracellular calcium concentration changes were recorded by using intracellular calcium determination method and cell proliferation was observed by MTT method in H9c2 cell. Results NP(1×10-10 mol/L)increased the intra⁃cellular calcium concentration changing amplitude and promoted the proliferation of H9c2 cells,while NP(1×10-6 mol/L)decreased intracellular calcium concentration changing amplitude and suppressed cell proliferation. G15 could block the promoting effect of 1×10-10 mol/L NP on the intracel⁃lular calcium concentration and cell proliferation,but could not block the inhibition of 1×10-6 mol/L NP on the intracellular calcium increase and cell proliferation. Conclusion The results indicate that NP affect rapid calcium signal changes and cell proliferation in non⁃monotonic dose dependent manner,and its mechanism may be due to the different involvement of GPR30 receptor in different concentrations.