rs3660单核苷酸多态性与非霍奇金淋巴瘤相关性研究

摘要

目的 评估KRT81基因的3’端非翻译区miRNA结合位点rs3660单核苷酸多态性(miR-SNP)对非霍奇金淋巴瘤(NHL)患者癌症风险和临床预后的影响.方法 采用连接酶检测rs3660 SNP分型;对数秩检验及Kaplan Meier方法分析rs3660与NHL生存相关性.采用Cox比例风险模型进行多因素生存分析.结果 rs3660基因分型在病例组及对照组中分布无统计学差异(P=0.50).患者携带rs3660 CG/CC基因型与GG基因型比较显示较长的生存时间(P=0.012).此外,多因素分析证明rs3660与NHL患者总体生存率独立相关(RR=0.589,95％CI:0.415～0.832,P=0.004).在外周T细胞淋巴瘤亚型中也观察到了这种miR-SNP对患者预后的影响价值.结论 KRT81 rs3660 GG基因型是NHL预后的独立预测标志.%Objective To evaluate the effect of single-nucleotide polymorphisms at the miRNA binding site rs3660 in the 3'-untranslated region of the KRT81 gene (miR-SNPs) on the cancer risk and clinical prognosis of non-Hodgkin's lymphomas (NHL).Methods The single-nucleo-tide polymorphisms of rs3660 was genotyped with ligation detection reaction method.The association of rs3660 with NHL survival was calculated with log-rank test using Kaplan-Meier method.Multivariate survival analysis was performed using a Cox proportional hazards model.Results The rs3660 genotype distribution difference was not statistically significant between the case and control group (P =0.50).Patients carrying the rs3660 CG/CC genotype exhibited a significantly longer survival time than patients carrying the GG genotype (P =0.012).In addition,rs3660 was associated independently with the survival of NHL patients in multivariate analysis (RR=0.589,95％ CI:0.415-0.832,P =0.004).The association of this miR-SNP with NHL survival was further confirmed in the peripheral T cell lymphoma (PTCL) subtype.Conclusion Our results indicate that KRT81 rs3660 GG type is an independent prognostic marker in NHL.