原发性卵巢功能不全小鼠卵巢血流与性激素浓度相关性研究

摘要

Objective To study the correlation between haemodynamics and sex hormone concentrations in mice with premature ovarian insufficiency (POI).Methods BALB/c female mice model of POI was established by multiple sites subcutaneous injection of zona pellucida 3 of mice,and then ovaries were observed by applying ultrasound meanwhile hemodynamics changes were recorded by ultrasound color doppler.Finally,mice serum was taken to be detected.Results Under the images of ultrasound,ovaries of mice in model group experience the volume decrease,follicle decrease and low blood flow.Compared to the blank group,mice of model group have lower pulsatility index (PI),higher resistance index (RI) and an ascending trend of pulse pressure (PP).Moreover,the folliclestimulating hormone (FSH) and anti-Müllerian hormone (AMH) in mice of model group had a significant difference (P ＜ 0.05) compared to the blank group.Then,through the correlation test between haemodynamics and sex hormone concentrations,there was a significant difference in PP and FSH (P ＜ 0.05).Conclusion The PP in haemodynamics and FSH in sex hormone concentrations have a significant difference and it can provide the base for dynamic observation in course of treatment.%目的 研究原发性卵巢功能不全(primary ovarian insufficiency,POI)小鼠卵巢的血流动力学与性激素浓度的相关性.方法 以小鼠透明带3作为抗原,皮下多点注射,免疫BALB/C雌性小鼠建立原发性卵巢功能不全模型,利用超声动态观察卵巢的形态,同时利用超声彩色多普勒成像观察卵巢的血流变化,最后取小鼠血清进行性激素浓度检测.结果 超声下可观察到模型组小鼠卵巢体积减小,卵泡数目减少,血流分布较弱.与空白组小鼠相比较,模型组小鼠阻力指数(resistive index,RI)较高,搏动指数(pulsatility index,PI,PI=S-D/Vmean)较低,脉压差(pulse pressure,PP)呈升高趋势;与空白组小鼠相比较,模型组小鼠的促卵泡生长激素(follicle-stimulating hormone,FSH)、抗苗勒管激素(anti-Müllerian hormone,AMH)均差异有统计学意义(P＜0.05).进一步通过模型组小鼠血流动力学与激素浓度相关性检验,发现模型组小鼠PP与FSH有一定(P＜0.05).结论 POI小鼠血流动力学PP与血清学FSH浓度有显著相关性,本研究为动态观察POI治疗过程提供参考.