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Expression, purification and characterization of Mycobacterium tuberculosis RpfE protein

机译:结核分枝杆菌RpfE蛋白的表达,纯化和鉴定

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摘要

Resuscitation promoting factor E(RpfE)is one of the five Rpf-like proteins in Mycobacterium tuberculosis(M.tuberculosis).These Rpf-like proteins are secretory,which make them candidates for recognition by the host immune system.In this study,the RpfE gene was amplified from M.tuberculosis,cloned into the expression vectors pDE22 and pPRO EXHT,and were expressed in Mycobacterium vaccae(M.vaccae)and Escherichia coli DH5α,respectively.Both recombinant RpfE proteins were purified by Ni-Sepharose affinity chromatography,and were given to C57BL/6 mice.The RpfE proteins elicited T cell proliferation,and stimulated the production of gamma interferon(IFN-γ),interleukin-10(IL-10)and IL-12.Our results indicated that the RpfE protein expressed in M.vaccae could more efficiently stimulate cellular immune response,making it a promising candidate as a subunit vaccine.

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  • 来源
    《生物医学研究杂志(英文版)》 |2012年第1期|17-23|共7页
  • 作者单位

    Department of Radiation Therapy, Xijing Hospital, the Fourth Military Medical University, Xian, Shaanxi 710032, China;

    Department of Microbiology, School of Basic Medicine, the Fourth Military Medical University, Xian, Shaanxi 710032, China;

    Department of Microbiology,School of Basic Medicine,the Fourth Military Medical University,Xian,Shaanxi 710032,China;

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  • 入库时间 2022-08-19 03:48:01
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