The remedial effect of soluble interleukin-1 receptor type Ⅱ on endometriosis in the nude mouse model

摘要

Objective:Recent studies have shown that the local expression of soluble interleukin(IL)-1 receptor typeⅡ (sIL-1 RⅡ)in endometrial tissue of women with endometriosis is decreased,and the depression of IL-1 RⅡwas more significant in infertile women than that in fertile women with endometriosis.In this research,we investigated the remedial effect of sIL-1-RⅡadministration on endometriosis in the nude mouse model.Methods: Nineteen nude model mice with endometriosis were randomly divided into three groups:group A was treated by intraperitoneal administration with only sIL-1 RⅡfor two weeks,group B was similarly treated with only IL- 1,and group C(control)was administered saline.After 2 weeks,the size of the ectopic endometrial lesions was calculated,and the expression of vascular endothelial growth factor(VEGF)and B-cell lymphoma leukemia-2(Bcl- 2)were detected by immunohistochemistry.The IL-8 and VEGF levels in the peritoneal fluid(PF)and serum were also measured by enzyme-linked immunosorbent assay(ELISA).Results:The mean size of ectopic endometrial lesion did not differ between the three groups(P>0.05).Compared with the control,the expression of VEGF and Bcl-2 was significantly lower in group A,and higher in group B.In the three groups,the levels of IL-8 in the PF and serum were highest in group A,and lowest in group B.Conclusion:sIL-1 RⅡmay suppresse hyperplasia of ectopic endometriosis,perhaps by reducing the expression of certain cytokines,such as VEGF,IL-8,and Bcl-2, which could provide a new clinical strategy for the treatment of endometriosis.