生物多样性与动物携带汉坦病毒相关性

摘要

为了解西安市肾综合征出血热的宿主动物群落特征与携带汉坦病毒的相互关系,以夹夜法捕鼠,记录鼠种和生存环境,无菌解剖后取鼠肺提取RNA,并进行病毒分型.采用BIOTOOLS分析了不同生境的生物多样性指数,SPSS分析了不同生境生物多样性指数与宿主动物带毒率的相互关系.结果显示:不同生境宿主动物生物多样性和带毒指数均差异显著(F=7.463,P<0.01;F=7.892,P<0.01),农田和撂荒地宿主动物带毒指数较高,山地生境未发现携带汉坦病毒动物;山地生境的生物多样性指数最高,显著高于农田和撂荒地;宿主动物带毒率与生物多样性指数Shannon-Wiener(H')、argalef(dMa)、Evenness(E)呈现显著负相关(R2=0.4255,P<0.01;R2=0.4368,P<0.01;R2=0.3321,P<0.05)、与Simpson's Dominance(λ)呈显著正相关.山地环境对宿主动物传播汉坦病毒的抑制作用明显,栖息地生物物种多样性丰富有利于群落结构稳定,有助于降低出血热的传播风险,物种多样性低且优势度明显的区域应当加强出血热的防控.%In order to understand the relationship between the characteristics of animal community and host animals virus-carrying con-dition of hemorrhagic fever with renal syndrome ( HFRS) , night trapping method was used to capture animals. We recorded the murids and their living environments, extracted RNA and genotyped of Hantavirus ( HV) carried by the murine-like animal. The biological di-versity in vary kind of habitats was analyzed by BIOTOOLS, and that the correlation between biodiversity and animal carrying HV was analyzed by using SPSS. The result showed that there was significant difference in the biological diversity as well as the index of virus carriage of host animal in different habitats(F=7. 463,P<0. 01;F=7. 892,P<0. 01). Indexes of virus carriage in farmland and wasteland were high, whereas the animal carrying HV was not found in mountain land;biodiversity index in mountain land was higher than that in farmland and wasteland. There was significantly negative correlation between virus-carrying rate of host animal and biodi-versity index(Shannon-Wiener(H'),Margalef(dMa) and Evenness(E)),(R2 =0. 4255,P <0. 01; R2 =0. 4368,P <0. 01; R2 =0. 3321,P<0. 05) whereas with which was positive correlation Simpson's Dominance(λ). The mountain land has a inhibitory effect on the transmission of HV among host animals, extreme abundant biodiversity is beneficial to the stability of animal community structure, which can help to reduce the risk of passing the virus on. The areas in which have low biodiversity community as well as have obvious dominance are dangerous for HFRS, we should strengthen the prevention and control.