瘦素抵抗肥胖雌性大鼠肺通气功能的损伤研究

摘要

目的:观察高脂饮食诱导的瘦素抵抗肥胖大鼠肺通气功能(PVF)的变化.方法:将30只SD雌性刚离乳大鼠按体重随机分成对照组和高脂组各15只;对照组给予普通饲料喂养,高脂组给予高脂饲料喂养,第8周末按体重增量从高脂组中筛选出7只大鼠作为瘦素抵抗肥胖组,测定2组大鼠的体重、血清瘦素浓度及潮气量(TV)、肺通气量(PV)、用力肺活量(FVC)、最大呼气中段流量(MMEF)、吸气峰流量(PIF)和呼气峰流量(PEF).结果:瘦素抵抗肥胖组第3周末至第8周末大鼠的体重和第8周末血清瘦素浓度、PV、PIF与PEF均高于对照组(P<0.05～P<0.01),TV、FVC 和MMEF差异均无统计学意义(P＞0.05).结论:饮食诱导的瘦素抵抗性肥胖降低了雌性大鼠的PVF.%Objective:To explore the changes of pulmonary ventilation function( PVF) in obese rats with leptin resistance induced by high-fat diet. Methods: Thirty female Sprague-Dawley weaning rats were randomly divided into control group and high-fat diet group according to their body weight. Fifteen rats were fed with basic diet and 15 rats were fed with high-fat diet. At the end of the 8th week, 7 obese rats were selected by body weight from high-fat diet group as obese group with leptin resistance. The body weight,blood serum leptin concentration, tidal volume ( TV ) , pulmonary ventilation ( PV ) , forced vital capacity ( FVC ) , maximal midexpiratary flow curve ( MMEF) ,peak inspiratory flow( PIF) and peak expiratory flow( PEF) of all rats in two groups were detected. Results:The body weight from the 3rd week to the 8th week,blood serum leptin concentration,PV,PIF and PEF of obese group at the end of the 8th week were higher than those of control group ( P < 0. 05 - P < 0. 01) . No significant difference of TV, FVC and MMEF were found between two groups at the end of 8th week. Conclusions: Obesity with leptin resistance induced by high-fat diet impairs the PVF of female rats.