金雀异黄酮对大鼠小脑浦肯野细胞缺血后动作电位编码的影响

摘要

目的 观察金雀异黄酮(genistein,GEN)对大鼠小脑浦肯野细胞缺血后动作电位编码的影响.方法 采用微电极技术和全细胞膜片钳方法,观察大鼠小脑浦肯野细胞在200 μmol/L GEN培养10 min后缺血时动作电位编码的变化.结果 缺血诱导大鼠小脑浦肯野细胞序列发放的动作电位峰间距缩短,动作电位峰时程标准差增大,细胞过度兴奋,动作电位编码的精确性降低.GEN能抑制脑缺血后序列发放的动作电位峰间距缩短及动作电位峰时程标准差显著增大.结论 GEN能预防小脑浦肯野细胞的过度兴奋,稳定缺血后细胞动作电位的编码能力.%Objective: To observe the influence of genistein on spike encoding of cerebellar Purkenje cells after ischemia. Methods: The microelectrode and whole cell voltage clamp technique were used in the experiment. The changes of spike encoding of cerebellar Purkenje cells were detected during the ischemia after the cells were incubated with genistein at 200μmol/L for 10 min. Results: After ischemia, the values of inter-spike interval of sequential spikes were shortened and the values of standard deviation of spike timing were increased, the cells were overexcited, the accuracy of spike encoding was decreased, which were prevented by genistein. Conclusions: Genistein can prevent ischemia-induced over-excitation and stabilize the spike encoding of cerebellar Purkinje cells after ischemia.