鲍曼不动杆菌脓毒症大鼠中性粒细胞中抗氧化蛋白6、丝氨酸蛋白酶抑制剂B1和翻译控制肿瘤蛋白表达变化

摘要

Objective:To explore the expressions of the Peroxiredoxin 6 ( PRDX6 ) , serine protease inhibitor B1 ( SERPIN B1 ) and translation control tumor protein translationally controlled tumor protein( TCTP) in neutrophils of Acinetobacter baumannii sepsis rats using Western blot technology at different points time. Methods:The Acinetobacter baumannii rat model was established by intraperitoneal injection with Acinetobacter baumannii. The total proteins of neutrophils in normal rats and rats after 6 and 12 h of sepsis were harvested. The protein levels of PRDX6 SERPIN B1 and TCTP in normal and sepsis rats were detected by western blot, and compared with the results of two-dimensional gel electrophoresis. Results:The PRDX6 expression levels in neutrophils of Acinetobacter baumannii rats after 6 and 12 h of sepsis were significantly lower than that of normal rats(P<0. 01),the increasing degree of which in rats after 6 h of sepsis was more significantly than that in rats after 12 h of sepsis(P<0. 01). The SERPIN B1 and TCTP expression levels in neutrophils of Acinetobacter baumannii rats after 6 and 12 h of sepsis were significantly higher than those of normal rats( P<0.01),the differences of which between sepsis rats after 6 and 12 h of sepsis were not statistically significant(P >0. 05). The expression levels of PRDX6 SERPIN B1 and TCTP were in accordance with the results of proteomics. Conclusions:The expressions of PRDX6、SERPIN B1 and TCTP in neutrophils of Acinetobacter baumannii sepsis rats are different from the normal rats,which can used as the target molecules in the early diagnosis and treatment of Acinetobacter baumannii.%目的：：通过蛋白印迹技术验证鲍曼不动杆菌脓毒症大鼠中性粒细胞中抗氧化蛋白6(PRDX6)、丝氨酸蛋白酶抑制剂B1(SERPIN B1)和翻译控制肿瘤蛋白(TCTP)在不同时间点的表达情况。方法：将18只清洁级雄性成年Wistar大鼠随机分为正常对照组(N组)、脓毒症6 h组(6 h组)和12 h组,每组6只。利用鲍曼不动杆菌腹腔注射制作鲍曼不动杆菌脓毒症大鼠模型,提取N组、6 h组和12 h组大鼠的中性粒细胞总蛋白,用免疫印迹实验检测PRDX6、SERPIN B1和TCTP的表达变化,并与前期双向凝胶电泳结果做对比分析。结果：6 h组和12 h组中性粒细胞中PRDX6表达量均明显低于N组(P<0.01),12 h组较6 h组下降更明显(P<0.01)；6 h组和12 h组SERPIN B1的表达量均高于N组(P<0.01),6 h组和12 h组差异无统计学意义(P>0.05)；6 h组和12 h组TCTP的表达量均显著高于N组(P<0.01),而6 h组和12 h组差异无统计学意义(P>0.05),3种蛋白的表达与前期蛋白质组学实验结果基本一致。结论：鲍曼不动杆菌脓毒症组大鼠中性粒细胞中 PRDX6、SERPIN B1和TCTP表达与正常组之间存在明显差异,PRDX6、SERPIN B1和TCTP可能会成为鲍曼不动杆菌脓毒症早期诊断和治疗的分子靶点。