不同类型HBV感染者T细胞对HBV抗原蛋白的免疫应答研究

摘要

Objective:To explore the T cell immune response function in different clinical manifestation patients. Methods:The peripheral blood mononuclear cells were isolated. The T cell subgroup was detected using flow cytometry,and the immune response of T cell to HBV antigen protein was detected using enzyme-linked immunespots assay. Results:Compared with the acute hepatitis patients (group A),the frequency of CD4 + T cell subgroup in chronic hepatitis patients(group B) and asymptomatic carriers(group C) were significantly lower(P0. 05). Compared with group A,the ratios of CD4 + /CD8 + in group B and group C were decreased(P0. 05);与A组比较,B组和C组CD4 + /CD8 +比值均明显下降(P<0. 01);A组斑点形成细胞显著少于B组和C组(P<0. 01);B组斑点形成细胞亦显著少于C组(P<0. 01). 结论:不同临床表现形式的患者T细胞免疫存在不同,而T细胞亚群之间的平衡失调可能导致机体免疫紊乱,不能清除病毒,是HBV感染患者表现不同临床症状的原因.