针刺预处理对大鼠小脑浦肯野细胞缺血性损伤的影响

摘要

目的：：研究针刺预处理百会穴+双侧足三里穴对脑缺血诱导的大鼠小脑浦肯野细胞损伤的影响。方法：将雄性SD大鼠30只随机分为正常对照组、针刺百会穴+双侧足三里穴组(针刺穴位组)和针刺非穴位组,各10只。采用糖氧剥夺的脑缺血模型,观察针刺预处理治疗后大鼠小脑浦肯野细胞的存活率,检测小脑组织中丙二醛( MDA)的含量和超氧化物歧化酶( SOD)的活性。结果：与正常对照组比较,针刺非穴位组大鼠脑缺血后浦肯野细胞死亡率明显增加,MDA水平明显增高,SOD活性明显降低(P<0.01)；与针刺非穴位组比较,针刺穴位组大鼠小脑浦肯野细胞存活率明显增加,小脑组织中MDA含量明显降低,SOD活性明显增加(P<0.01)。结论：针刺百会穴+双侧足三里穴对大鼠小脑浦肯野细胞缺血性损伤具有一定的保护作用,其机制可能与小脑组织中活性氧的生成减少和清除增加有关。%Objective:To investigate the influence of acupuncture pretreatment Baihui-point and bilateral Zusanli-point on ischemia-induced impairment of cerebellar Purkenje cells. Methods:Thirty male SD rats were randomly divided into three groups:control group, acupuncture pretreatment in Baihui-point and bilateral Zusanli-point group and pretreatment in non acupuncture point group,10 rats in each group. The oxygen-glucose deprivation model was established,the survival rate of Purkinje cells,malondiadehyde( MDA) content and superoxide dismutase( SOD) activity were detected during the ischemia period after the rats were acupunctured. Results:Compared with the control,the survival rate of Purkinje cells was lower,MDA content was increased and SOD activity was decreased significantly in non-acupuncture pretreatment group(P<0. 01). Compared with the non-acupuncture pretreatment group,the survival rate of Purkinje cells was increased,MDA content was decreased,and SOD activity was increased in the acupuncture pretreatment in Baihui-point and bilateral Zusanli-point group(P<0. 01). Conclusions:Acupuncture pretreatment to Baihui-point and bilateral Zusanli-point prevented ischemia-induced functional impairment of cerebellar Purkenje cells,the protective mechanism may be related to reduce the production of reactive oxygen species and promote the clearance in cerebellar Purkenje cells.