内侧半月板部分切除联合前交叉韧带切断的大鼠膝关节炎模型研究

摘要

目的:采用内侧半月板部分切除加前交叉韧带切断的方法建立大鼠骨关节炎(OA)模型,观察模型大鼠膝关节病理变化进程.方法:选取SD雌性大鼠20只,采用手术切除内侧部分半月板和切断右膝关节前交叉韧带,左侧对照,术后第1、2、4、6、8周处死大鼠,取全膝,观察膝关节软骨外形、软骨结构、关节滑膜ED1阳性细胞数和影像学改变.结果:术后第1周开始出现软骨细胞肿胀,表层带软骨细胞死亡,数量减少,软骨基质减少,表层带纤维化等软骨损害;滑膜单克隆抗体ED1阳性渗出性细胞明显增多;4周后肉眼观察可见全膝软骨外形明显改变,软骨下骨密度增高及滑膜增生纤维化等变化.结论:采用切除SD大鼠膝关节内侧部分半月板加切断前交叉韧带能早期诱导产生OA的病理变化,是较理想的研究早期OA的动物模型.%Objective:To establish the osteoarthritis model by medial meniscus partial resection combined with anterior cruciate ligament transecting,and observe the pathological changes of knee joint in rats. Methods:Twenty female SD rats were implemented with medial meniscus partial resection combined with anterior cruciate ligament transecting in right knee joint,and the left side was set as the control. The whole knee joint was harvested after 1,2,4,6 and 8 weeks of operation,and the knee morphology,joint cartilage structure, synovial ED1-positive cells and X-ray image in knee joints were observed. Results:After 1 week of operation,cartilage cellular swelling, superficial cartilage cells death and decreasing,superficial fibrosis and number of monoclonal antibody ED1 positive cells in synovium increasing were detected. After 4 weeks of operation,the total knee morphological change,bone density under cartilage increasing and synovial hyperfibrosis were observed. Conclusions:The medial meniscus partial resection combined with anterior cruciate ligament transecting can early induce the pathological changes of OA,which is an ideal animal model in osteoarthritis study.