宁夏地区老年人群载脂蛋白E等位基因ε4携带与血脂水平的相关性研究

摘要

目的:分析老年人群中载脂蛋白E(APOE)基因多态性与血脂之间的关系.方法:采用典型抽样法抽取宁夏地区城市社区55岁及以上人群,行问卷调查和血脂相关指标检查;采用高通量溶解曲线法检测APOE基因多态性,APOE基因多态性与血脂关系采用方差分析.结果:共924人完成了所有研究内容;各年龄组三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、载脂蛋白A(ApoA)、体质量指数(BMI)、葡萄糖5项指标差异有统计学意义(P<0.05);等位基因频率分布为E3所占比例最大,且不同年龄组间、不同性别间基因型分布、等位基因频率分布、携带基因E4频率分布差异均有统计学意义(P<0.05),将年龄和性别进行控制后,基因型与HDL-C、LDL-C、ApoA和ApoB均有相关关系(P<0.05~P<0.01).结论:宁夏地区老年人群的血脂水平分布与年龄存在相关性,等位基因ε4携带与血脂水平显著相关.%Objective:To analyze the correlation between the apolipoprotein E( ApoE) gene polymorphism and blood lipid in elders. Methods:Nine hundred and twenty-four elders aged over 55 years old were selected using typical sampling method. The cases were investigated using a questionnaire,and the blood lipid indicators were examined. The ApoE gene polymorphism was detected using the high resolution melting( HRM) curve method. The correlation between ApoE gene polymorphism and blood lipid was analyzed using ANOVA. Results:The differences of the levels of TG,HDL-C,ApoA,BMI and glucose in all cases were statistically significant( P<0. 05). The proportion of E3 in all allele frequency distribution was the largest. The differences of the genotype distribution,allele frequency distribution and carry E4 frequency distribution among all cases were statistically significant(P<0. 05). After the age and gender were controlled,the genotype was correlation with the HDL-C,LDL-C,ApoA and ApoB(P<0. 05 to P<0. 01). Conclusions:The blood lipid level is associated with age,and the ApoE4 carriers is significant associated with the blood lipid level of the elders in Ningxia.