不同时机介入电针对模拟失重大鼠肝脏组织结构、功能和一氧化氮水平的影响

摘要

目的：观察预针刺和针刺治疗对模拟失重大鼠肝脏组织结构、功能和一氧化氮（NO）水平的影响，探讨不同时机介入电针治疗对模拟失重大鼠肝脏损伤的防护机制。方法将20只雄性清洁级 Wistar 大鼠随机分为正常组、模型组、预针刺组和针刺组，每组5只。正常组不做任何处理，自由活动饲养4周；模型组、预针刺组和针刺组大鼠尾部悬吊4周制造模拟失重大鼠模型，预针刺组在尾吊前1周，电针刺激双侧“肾俞”“脾俞”和“三阴交”穴，每次30 min，每日1次，共治疗7次；针刺组针刺治疗与尾部悬吊同时开始，大鼠尾部悬吊过程中，电针刺激“肾俞”“脾俞”和“三阴交”穴，每次30 min，隔日治疗1次，共针刺14次。采用 HE 染色法物镜下观察各组大鼠肝脏组织结构的变化，比色法检测各组大鼠血清谷丙转氨酶（ALT）活性及肝脏组织一氧化氮（NO）含量。结果肝脏组织 HE 染色切片观察显示：模型组大鼠肝脏中央静脉周围出现大面积的肝细胞变性；经电针治疗后，大鼠肝脏组织结构病理变化较模型组有所改善，且预针刺组疗效优于针刺组。与正常组比较，模型组大鼠血清 ALT 活性和肝脏组织 NO 含量显著升高（P ＜0．05，P ＜0．01）；与模型组比较，预针刺组大鼠血清 ALT 活性及肝脏组织 NO 含量显著降低（均 P ＜0．05），针刺组以上指标变化不显著。与预针刺组比较，针刺组 ALT 活性有升高趋势，NO 含量显著升高（P ＜0．01）。结论预针刺较针刺治疗更能有效地缓解大鼠尾部悬吊引起的肝脏组织结构和功能的损伤，其机理可能与NO 有关。%Objective To observe the effect of needling on the structure,function and level of nitric oxide (NO)of the liver in rats with simulated weightlessness,so as to explore the protective mechanism of acu-puncture on liver injury.Methods Twenty male clean grade Wistar rats were randomly divided into nor-mal group,model group,pretreatment group and treatment group(each n =5).The rats of normal group received no treatment,and those of other three groups were tail -suspended for 4 weeks to establish weightlessness model.Electroacupuncture at the points of Shenshu (BL23),Pishu (BL20)and Sanyin-jiao (SP60)once daily for 30 min was applied one week ahead of tail-suspension in pre-treatment group, while in treatment group the needling treatment applied at the same three points once every other day for 14 d was carried out at the day when the rats was tail -suspended.The changes of hepatic histological structure were observed,and the activity of alanine aminotransferase(ALT)in serum and the content of NO in liver were measured.Results The hepatic cell degeneration were observed around the central vein in model group;the degree of degeneration relieved in both electroacupuncture treatment groups.Further-more,the curative effect of the pre-treatment group was better than the treatment group.Compared with normal group,the activity of ALT in serum and the content of NO in liver in model group increased signif-icantly (P <0.05,P <0.01).Compared with model group,the activity of ALT in serum and the con-tent of NO in liver in the pre-treatment group significantly reduced(P <0.05),however,those in treat-ment group had no obvious difference.Compared with pre-treatment group,the activity of ALT tended to increase and the content of NO increased significantly(P <0.01)in the acupuncture group.Conclusion The effects of relieving hepatic injury and dysfunction induced by weightlessness,which potentially was related to the content of NO,in the pre-treatment group were superior to those in the treatment group.