目的：对小叶金露梅不同提取部位的α-葡萄糖苷酶（ AG）和醛糖还原酶（ AR）抑制活性进行研究，找寻天然的AG和AR抑制剂。方法体外实验通过α-葡萄糖苷酶抑制剂和醛糖还原酶抑制剂筛选模型，对小叶金露梅乙酸乙酯部位（总黄酮）、石油醚部位和水层部位提取物的AG和AR抑制活性进行测定；体内实验进行小叶金露梅总黄酮对小鼠糖耐量的测定。结果小叶金露梅总黄酮部位对AG和AR有较高的抑制活性，而且总黄酮部位抑制AG活性的作用强于阿卡波糖。同时，小叶金露梅总黄酮高、低剂量均能提高小鼠的糖耐量。结论小叶金露梅总黄酮对α-葡萄糖苷酶和醛糖还原酶具有抑制活性。%Objective To study the inhibitory effects on α-glucosidase ( AG ) and aldose reductase ( AR) extracted from Potentilla parvifolia in different solvents , and to find out natural inhibitors of AG and AR.Methods With a combined vitro and vivo experiment design , this study measured the in vitro inhibitory effects of AG and AR extraction from Potentilla Parvifolia in three solvents:ethyl acetate ( total flavonoids ) , petroleum ether and water through AG inhibitors and AR inhibitory activity screening mod-els .In vivo effects of total flavonoids from Potentilla Parvifolia on impaired glucose tolerance of mice were also measured .Results Extraction of Potentilla Parvifolia in total flavonoids demonstrated a stronger in-hibitory effect on AR and AG .Its inhibitory effect on AG was higher than that of acarbose .Meanwhile , both high and low dose of total flavonoids improved glucose tolerance in mice .Conclusion Total fla-vonoids in Potentilla parvifolia exhibits inhibitory effects on AG and AR .
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