表没食子儿茶素没食子酸酯和牛磺酸联用对犬白内障模型晶状体上皮细胞凋亡的影响

摘要

This research aims to provide a theoretical basis for veterinary clinical treating cateract with drugs,15 adult dogs of 5-10 kg were selected (30 eyes)and divided randomly into five groups: model,control,epigallocatechin gallate (EGCG),tau-rine and EGCG combined taurine,six replicates in each group.Intracystic injection of fenton solution with the except of control group.The lens turbid were checked with slit lamp microscope (SLM)and then graded using the lens opacities classification system Ⅲ(LOCⅢ).The models were treated with drugs when their lens turbid over II.40 days after treatment,the lens were taken out to observe the pathological changes by transmission electron microscopy (TEM),measure the expression of Bcl-2,Bax,Caspase-3 gene of lens epithelia with immune histochemistry (IHC)and quantitative real-time PCR (qRT-PCR).The results showed that compared with the model group,the ratio of Bax/Bcl-2,and the expression of Caspase-3 of the combined group were significantly decreased (P<0.05).The combined medicine could up-regulated the expression of the apoptosis gene of Bcl-2 to inhibit apoptosis.%为提供兽医临床用药治疗白内障的理论依据,选取健康成年犬15只(30眼),随机分为模型组、表没食子儿茶素没食子酸酯(EGCG)组、牛磺酸、EGCG联合牛磺酸组和对照组,每组6个重复.除对照组外其他组用fenton液造模.裂隙灯观察晶状体的混浊情况,并根据国际通用的晶状体混浊分类体系Ⅲ(LOCSⅢ)分类,当混浊达到Ⅱ期时用药治疗.临床用药40 d后,取出晶状体透射电镜观察形态结构的变化；免疫组织化学法测定晶状体上皮细胞Bcl-2、Bax和Caspase-3蛋白表达水平；qRT-PCR测定其mRNA的表达水平.结果表明,与模型组相比联合组中Bax/Bcl-2的比值和Caspase-3的表达较模型组显著降低(P＜0.05),证明联合用药可以通过上调凋亡基因Bcl-2的表达抑制细胞凋亡.