Study on metabolites of bioactive compounds assisted by LC-SPE-NMR

摘要

Aim To study the metabolites of active compound dicentrine in miniature pig.Method Solvent extraction and column chromatographic methods were used to give the metabolites containing fractions of pig urine.UV absorption,mass spectrometry and HPLC-SPE-NMR were used to identify the metabolites of dicentrine.Results Twenty-four in total,including nine phase I metabolites(MI-1～9) and 15 phase II metabolites(MII-1～15),were characterized.Conclusion Dicentrine easily metabolizes into all kinds of compounds structurally related to pig body.