Identification and quantitative mRNA analysis of a novel splice variant of GPIHBP1 in dairy cattle

摘要

Background:Identification of functional genes affecting milk production traits is very crucial for improving breeding efficiency in dairy cattle.Many potential candidate genes have been identified through our previous genome wide association study(GWAS).Of these,GPIHBP1 is an important novel candidate gene for milk production traits.However,the mRNA structure of the bovine GPIHBP1 gene is not fully determined up to now.Results:In this study,we identified a novel alternatively splice transcript variant(X5) which leads to a 31 bp insertion in exon 3 and also confirmed the other four existed transcripts(X1,X2,X3 and X4) of the bovine GPIHBP1 gene.We showed that transcript X5 with a 31 bp insertion and transcript X1 with an 8 bp deletion might have tremendous effect on the protein function and structure of GPIHBP1,respectively.With semi-quantitative PCR and quantitative real-time RT-PCR,we found that the mRNA expression of GPIHBP1,GPIHBP1-X1 and GPIHBP1-X5 in mammary gland of lactating cows were much higher than that in other tissues.Conclusions:Our study reports a novel alternative splicing of GPIHBP1 in bovine for the first time and provide useful information for the further functional analyses of GPIHBP1 in dairy cattle.