降解甲醛的假单胞菌菌株的固定化研究

摘要

从印染厂采集的活性污泥中筛选得到1株快速降解甲醛的菌株并命名为 W1,通过形态与生理生化特征的鉴定,初步鉴定 W1菌株为假单胞菌属(Pseudomonas)．以海藻酸钠为包埋载体固定 W1菌株进行降解甲醛的初步研究,采用不同海藻酸钠浓度、菌悬液添加量配制成不同的包埋载体,利用 L9(33)正交试验对 W1菌株降解甲醛条件进行优化,分析其甲醛降解率的变化．实验结果表明：培养基为(NH4)2 SO42．4 g/L, MgSO4•7H2 O 0．2 g/L,微量元素母液0．1 mL,pH 值9．0,30℃恒温培养,在此条件下甲醛48h内的降解率达80．3％．通过对甲醛降解菌 W1固定化的研究,为生物法去除甲醛的应用奠定基础．%A formaldehyde-degrading bacterium strain was screened from the activated sludges,which was named W1 .The strain was preliminarily identified as Pseudomonas according to its morphology and bio-chemical test.Meanwhile,the growth and degradation characteristics of strains W1 were preliminarily studied with entrapped in sodium alginate as the carrier.W1 was immobilized with sodium alginate of different concentrations and added with different bacterial suspension.The optimum conditions of formal-dehyde degradation efficiency by L9 (33 )orthogonal experimental design were as follows:concentration of (NH4)2SO4 2.4 g/L,MgSO4•7H2O 0.2 g/L,mother liquors of trace elements of 0.1 mL,pH value of 9.0,incubation temperature of 30 ℃.The results showed that the removal efficiency of formaldehyde of strain W1 could be as high as 80.3% in 48 h under optimal conditions.The experiment established the basis for the application of biological removal of formaldehyde based on the technique of immobilized mi-croorganism.