脑络欣通对大脑中动脉阻塞再灌注模型大鼠神经干细胞增殖分化的影响

摘要

目的 观察脑络欣通对大脑中动脉阻塞再灌注(middle cerebral artery occlusion-reperfusion,MCAO-R)模型大鼠神经干细胞增殖分化的影响.方法 采用线栓法复制MCAO-R大鼠模型,将30只健康雄性SD大鼠随机分成假手术组、模型组和给药组.分别采用神经功能评分和TTC染色鉴定模型大鼠的神经功能缺损和脑缺血面积,采用免疫荧光染色法观察大鼠海马CA1、CA2、CA3、齿状回(dentate gyrus,DG)区巢蛋白(Nestin)和神经丝蛋白-H (hypophosphorylated neurofilament-H,NF-H)表达水平.结果 给药组Nestin阳性表达主要发生在DG区,NF H阳性表达主要发生在CA3区.脑络欣通促进神经干细胞增殖的作用在DG、CA1、CA2、CA3区均十分明显,以DG区尤为突出,促进神经干细胞分化的作用主要表现在CA3、DG、CA2、CA1区.与模型组比较,给药组大鼠海马各区的Nestin、NF-H阳性细胞均明显增多,差异具有统计学意义(P＜0.05).结论 脑络欣通具有促进海马区神经干细胞增殖并分化为神经细胞的作用.%Objective To investigate the effect of Naoluoxintong on the proliferation and differentiation of neural stem cells in a rat model of middle cerebral artery occlusion/reperfusion (MCAO/R).Methods The suture method was used to establish a rat model of MCAO/R,and a total of 30 healthy male Sprague-Dawley rats were randomly divided into sham-operation group,model group,and administration group.The neurological score was used to evaluate neurological defects,TTC staining was used to measure the area of cerebral ischemia,and immunofluorescent staining was used to observe the expression of Nestin and hypophosphorylated neurofilament-H (NF-H) in the CA1,CA2,CA3,and dentate gyrus (DG) regions of the hippocampus.Results In the administration group,Nestin was mainly expressed in the DG of the hippocampus and NF-H was mainly expressed in the CA3 region of the hippocampus.Naoluoxintong significantly promoted the proliferation of neural stem cells in the CA1,CA2,CA3,and DG regions of the hippocampus,especially in the DG region,and it significantly promoted the differentiation of neural stem cells in the CA3,DG,CA2,and CA1 regions.Compared with the model group,the administration group had significant increases in the numbers of cells with positive Nestin and NF-H in each region of the hippocampus (P＜0.05).Conclusion Naoluoxintong can promote the proliferation of neural stem cells and the differentiation of such cells into neural cells in the hippocampus.