芪黄煎剂对胃切除大鼠肠淋巴细胞MAdCAM-1、ICAM-1及其mRNA表达的影响

摘要

Objective To investigate the effect of intra-small intestinal drip of Qihuang Decoction on the protein and mRNA expression of mucosal addressin cell adhesion molecule-1 (MAdCAM-1) and intercellular adhesion molecule-1 (ICAM-1) in rats after gastrectomy.Methods A total of 60 Sprague-Dawley rats were divided into three groups,with 20 rats in each group.The rats in sham-operation group were treated with abdominal skin incision and suture,those in model group were given enteral nutrient solution (Nutrison) after gastrectomy,and those in Qihuang Decoction group were given Qihuang Decoction followed by Nutrison after gastrectomy.After 7 consecutive days of administration,immunohistochemistry was used to measure the protein expression of MAdCAM-1 and ICAM-1,and RT-PCR was used to measure the relative mRNA expression of MAdCAM-1 and ICAM-1.Results Compared with the sham-operation group,the model group had significant reductions in the protein and mRNA expression of MAdCAM-1 and ICAM-1 in the Peyer's patch (P＜0.05),and the Qihuang Decoction group had significant increases in the protein and mRNA expression of MAdCAM-1 and ICAM-1 (P＜0.05).Conclusion Qihuang Decoction can increase the protein and mRNA expression of MAdCAM-1 and ICAM-1 at the intestinal mucosal effector sites and improve the immunological barrier function of the intestinal mucosa after gastrectomy.%目的 观察大鼠胃切除术后小肠内滴注芪黄煎剂对黏膜地址素细胞黏附分子-1 (mucosal addressin cell adhension molecule-1,MAdCAM-1)与细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)及其mRNA表达的影响.方法 将60只SD大鼠分为3组,每组20只.假手术组大鼠仅行腹部皮肤切开后缝合手术,模型组大鼠胃切除后给予肠内营养液能全素,芪黄煎剂组大鼠胃切除后先给予芪黄煎剂再滴注营养液能全素.连续给药7d后,采用免疫组织化学法检测MAdCAM-1、ICAM-1蛋白表达水平,采用RT-PCR荧光定量法检测MAdCAM-1、ICAM-1mRNA相对表达水平.结果 与假手术组比较,模型组大鼠PP结中MAdCAM-1、ICAM-1及其mRNA表达水平均明显下降(P＜0.05);与假手术组比较,芪黄煎剂组大鼠PP结中MAdCAM-1、ICAM-1及其mRNA表达水平明显升高(P＜0.05).结论 芪黄煎剂能增加肠黏膜效应部位MAdCAM-1、ICAM-1及其mRNA表达水平,改善胃切除术后肠黏膜免疫屏障功能.