Nicotinic Acetylcholine Receptor Gene Family of the Pea Aphid, Acyrthosiphon pisum

摘要

cqvip:The nicotinic acetylcholine receptors(nAchRs) are cholinergic receptors that form ligand-gated ion channels by five subunits in insect and vertebrate nervous systems.The insect nAChR is the molecular target of a class of insecticides,neonicotinoids.Here,we identified and cloned 11 candidate nAChR subunit genes in Acyrthosiphon pisum using genome-based bioinformatics combined modern molecular techniques.Most A.pisum nAChRs including 1,2,3,4,6,8,andβ1 show highly sequence identities with the counterparts of other insects examined.Expression profiles analysis showed that all subunit genes were expressed in adult head.At least two subunits have alternative splicing that obviously increase A.pisum nicotinic receptor diversity.This study will be invaluable for exploring the molecular mechanisms of neonicotinoid-like insecticides in sucking pests,and for ultimately establishing the screening platform of novel insecticides.