Transcripts of chalcone synthase (CHS) gene in six lines of Arabidopsis thaliana in response to UV-B radiation and treatment of hydroxyl peroxide(H2O2), D/L-NG-monomethyl-arginine(D-NAME, L-NAME) and 2-phenyl-4,4,4,4-tetramethylimidazolin-1-oxyl-3-oxide (PTIO) were studied by RT-PCR method. Mutant Fah1-2 was the most sensitive and wild WS2 was the most tolerant to UV-B damage in the six lines ofArabidopsis. Accumulation of transcripts of CHS gene was markedly elevated at second hour but disappeared after 24 h of 15 μmol/m2/s UV-B. H2O2 had not effect on the transcription of CHS gene. D-NAME and L-NAME suppressed the increase of CHS transcription in response to UV-B radiation while the effect of PTIO on CHS was very weak. It appears that CHS transcription in response to UV-B radiation is not regulated by nitric oxide(NO) and H2O2.%应用RT-PCR技术研究了6个拟南芥(Arabidopsis thaliana)株系的查耳酮合成酶(CHS)基因转录对UV-B照射以及过氧化氢(H2O2)、D/L-NG-一甲基精氨酸(D-NAME、L-NAME)和2-苯基-1-4,4,4,4-四甲基咪唑啉-1-烃氧基-3-氧化物(PTIO)等化合物处理的响应.结果表明,在6个株系中,Fah1-2突变株UV-B伤害最为敏感,而野生型WS2对UV-B伤害耐受力最强;在15μmol/m2/s UV-B照射2 h,CHS基因转录产物明显增加,但24 h后消失.H2O2对CHS基因转录没有显著作用,D-NAME和L-NAME能抑制CHS在UV-B照射下表达转录的增加,而PTIO对CHS基因表达的抑制作用很弱.据此认为,CHS基因对UV-B照射响应的表达和转录不受NO和H2O2的调节.
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