奶牛B2M基因单倍型组合与乳腺Fc受体(FcRn)mRNA表达丰度的相关分析

摘要

为研究奶牛B2M基因多态性与FcRn相对表达丰度之间的相关性以提高乳中IgG的转运量.本研究采集40头健康中国荷斯坦奶牛的乳腺组织样本,提取组织DNA后特异性扩增B2M基因片段,回收测序,根据测序结果寻找SNPs位点并分型,提取乳腺组织总RNA并反转录为cDNA,再利用Real-time PCR方法检测FcRn mRNA表达丰度.结果发现了3个B2M基因SNPs位点,能组成4种单倍型组合,其中单倍型组合H4的FcRn mRNA表达丰度最高,显著高于其他3种单倍型组合(P＜0.05),但单倍型组合H1,H2和H3之间差异均不显著(P＞0.05).B2M基因单倍型组合能影响奶牛乳腺FcRn mRNA表达丰度.%The study is conducted to investigate the association of B2M polymorphism with FcRn expression, in hope of improving the transportation of IgG in milk. The mammary gland samples were collected from 40 healthy Chinese Holstein cows immediately after slaughter and preserved in liquid nitrogen. We extracted total DNA from the frozen samples and designed specific primers to amplify B2M gene, followed by SNPs identification according to the sequencing results. The total RNA was purified from animal tissues according to the haplotypes of B2M, and then was transcripted reversely into cDNA. And the level of FcRn mRNA expression was detected by Real-time PCR. The results showed that there were three SNPs in B2M gene, which assembled four haplotype combinations.The expression of FcRn mRNA in H4 was significantly higher than that of the others (P＜0.05), but there were no significant differences (P＞0.05) between H1, H2 and H3. We conclude that the haplotype combinations of B2M have the effect on the expression of FcRn mRNA.