Effect of Electro-Acupuncture on S-100 Protein Expression of Nerve Fibers in the Repair Process of Transected Sciatic Nerve in Rats

摘要

Objective: To observe the effects of electro-acupuncture on S-100 protein expression of nerve fibers on the regeneration process of transected sciatic nerve of rats, so as to explore the partial mechanism of electro-acupuncture treatment for peripheral nerve injury.Methods: Following rat's sciatic nerve transection, the nerve regeneration chamber model was established, then the rat was given electro-acupuncture stimulation at the frequency of 5 Hz and frequency of 100 Hz, and the immunohistochemical staining method and image analyzing semi-quantitative technique were adopted to determine the percentage of S-100 protein area,hence to observethe effect of electro-acupuncture on the repair process of transected sciatic nerve of rat and the S-100 protein expression. Results: Electro-acupuncture could significantly potentiate the S-100 protein expression of Schwann Cells (SC) in the repair process of transected sciatic nerve. At the 14th week, 17th week and 20th week after operation, the percentages of S-100 protein area were more obviously increased in 5 Hz electro-acupuncture group and 100 Hz electro-acupuncture group than in model group, with significant differences (P＜0.05). Especially, the better effect was obtained in 5 Hz electro-acupuncture group. Judging from the dynamic treatment, it is very vital to be treated early. Conclusion: Electro-acupuncture is an effective method for the treatment of peripheral nerve injury, and its therapeutic effect is related to the improvement of SC proliferation.%目的:观察电针对大鼠坐骨神经截断后再生过程中神经纤维S-100蛋白的影响,旨在揭示电针治疗周围神经损伤的部分机理.方法:采用神经截断性损伤后构建神经再生室模型的方法,5 Hz和100 Hz电针刺激,应用免疫组化染色方法和图像分析半定量测定方法,测定S-100蛋白的面积百分比,观察电针对大鼠坐骨神经截断性损伤修复过程与S-100蛋白的表达情况.结果:电针能明显促进坐骨神经截断后修复过程中雪旺细胞(Schwarm Cells,SC)S-100蛋白表达,14星期、17星期和20星期SC S-100蛋白表达面积百分比5 Hz组、100 Hz组较模型明显增加,有显著性差异(P＜0.05),其中以5 Hz电针组治疗效果最佳,从动态治疗看,早期治疗十分重要.结论:电针是治疗周围神经损伤的重要手段,而且其疗效与促进SC增殖有关.