Influence of Moxibustion Serum on the Expression of Fas bcl-2 mRNA and Protein of EL-4 Lymphoma Cells

摘要

目的:观察小鼠艾灸血清体外对EL-4淋巴瘤细胞Fas、bcl-2mRNA及蛋白表达的影响.方法:采用小鼠艾灸血清培养EL-4淋巴瘤细胞24h后,应用原位杂交方法检测EL-4细胞Fas、bcl-2mRNA的表达,免疫细胞化学方法观察Fas、bcl-2蛋白表达.结果:与正常小鼠血清比较,艾灸血清培养EL-4细胞后,在bcl-2 mRNA及蛋白水平上,阳性细胞百分率均明显降低(P<0.05),在Fas mRNA及蛋白水平上,阳性细胞百分率均明显升高(P<0.05).结论:艾灸血清能够下调EL-4细胞bcl-2 mRNA及蛋白的表达,上调Fas mRNA及蛋白的表达.%Objective: To observe the influence of moxibustion sertma of mice on the expression of Fas, bcl-2 mRNA and protein of EL-4 lymphoma cells in vitro. Method: The EL-4 lymphoma cells were cultivated for 24 h in the moxibustion serum of mice. The expression of Fas and bcl-2 mRNA of EL-4 lymphoma cells were detected by in-situ hybridization method, and the protein expression of Fas and bcl-2 were observed by the immuocytochemistry method. Results: The expression of bcl-2 mRNA and protein decreased, and the expression of Fas rnRNA and protein increased significantly in EL-4 cells, which were cultivated in the moxibustion serum compared those cultivated in normal mice serum (P<0.05). Conclusion: Moxibustion serum could down-regulate the bcl-2 mRNA and protein and up-regulate the Fas mRNA and protein of EL-4 cells.