大鼠早期放射性脑损伤中星形胶质细胞的病理学改变

摘要

目的通过对脑组织中星形胶质细胞病理学改变的观察，研究放射性脑损伤的发病机理。方法利用已建立的大鼠早期放射性脑损伤的实验模型，用胶质纤维酸性蛋白免疫组织化学染色标记星形胶质细胞的方法，观察大脑海马区中星形胶质细胞形态与数量的变化，并分析照射剂量、照射后时间间隔的影响。结果受照射侧海马中的阳性细胞表现出胞体肥大、突起增多与延长等改变，在剂量增加和观察时间延长的条件下，20Gy照射后3个月和30Gy照射后1个月与3个月的三个组别中阳性细胞数有显著增加。结论随着照射剂量的加大和观测时间的延长，在大鼠大脑海马区域内出现了明显的星形胶质细胞增生。%Objective　To investigate the pathogenesis of brain radiation injury by observing the pathological changes of astrocytes in the hippocampus region of rat brain radiation injury model．Methods　The brain sections were immunohistochemical stained for glial fibrillary acidic protein （GFAP），which was used to label astrocytes．The morphological characters and the number of GFAP cells in hippocampus were recongnized，and the data were analyzed by the Student’s test．Results　The astrocytes proliferation was revealed．The relative number of GFAP positive cells increased in the hippocampus．The increase was dose and time course related．Conclusion　The pathological changes of astrocytes suggest a possible association in the pathogenesis of brain radiation injury，just as the other tissue components．