目的 探讨胰岛素样生长因子结合蛋白2(IGFBP-2)在脂肪肝组织中的表达及意义.方法 60只清洁级雄性SD大鼠随机均分为6组:模型组分别用四氯化碳(CCl4)、酒精、高脂饮食诱导,相应的对照组予以正常饮食.采用半定量免疫组织化学方法 检测脂肪肝大鼠肝脏组织的IGFBP-2表达.结果 三个脂肪肝模型组大鼠肝脏组织IGFBP-2表达均高于各自的对照组[(245.3±67.6)个/mm2vs.(53.5±15.3)个/mm2,(282.4±58.6)个/mn2 vs.(61.2±14.2)个/mm2,(352.8±81.3)个/mm2vs.(66.2±13.7)个/mm2](P＜0.01),且高脂模型组大鼠肝脏组织IGFBP-2表达高于CCl4模型组和酒精模型组大鼠(P＜0.05).结论 IGFBP-2可能参与脂肪肝发生发展,尤其与非酒精性脂肪肝的形成具有密切联系.%Objective To investigate the expression and significance of insulin-like growth factor binding proteir-2(IGFBP-2) in liver tissue of rat with fatty liver. Methods Sixty male SD rats were equally divided into 6 groups of three model groups and three corresponding control groups. The rats in model groups were induced fatty liver with CCl4, alcoholic or fat-rich diet. The expressions of IGFBP-2 in liver tissues were detected by semiquantitative immunohistochemistry. Results The expressions of IGFBP-2 in liver tissues of three model rats were significantly higher than those of corresponding control groups [(245. 3±67. 6) pieces/mm2 vs. (53. 5±15. 3) pieces/mm2, (282. 4±58. 6) pieces/mm2 vs (61. 2 ± 14. 2) pieces/mm2, (352.8 ± 81.3) pieces/mm2 vs. (66. 2 ± 13. 7)pieces/mm2] (P＜0.01),which were higher in high fat diet model group than those in CCl4 model group or alcoholic model group(P＜0. 05). Conclusion IGFBP2 may play an important role in the development of fatty liver, especially in the pathogenesis of non-alcoholic fatty liver.
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