大肠腺癌中微血管密度和Ki67表达及其临床意义

摘要

目的 探讨微血管密度(MVD)和Ki67在大肠腺癌组织中的表达及其临床意义.方法 用组织芯片和免疫组织化学的方法 检测115例大肠腺癌组织、19例大肠腺瘤组织和12例正常大肠黏膜组织中MVD与Ki67的表达,并分析其与临床病理特征的关系.结果 CD31和CD34标记的MVD差异无统计学意义;在大肠腺癌组织中,CD31和CD34标记的MVD计数分别为28.30±13.90和28.73±18.90,显著高于正常大肠黏膜组织的8.17±3.93和10.42±7.32,且MVD的表达与大肠癌的淋巴结转移、Dukes分期相关(P＜0.05);Ki67在正常大肠黏膜表达阳性率16.67%,明显低于腺瘤和腺癌中的31.58%和52.17%,且与Dukes分期明显相关(P＜0.01).MVD与Ki67的表达呈正相关.结论 大肠腺癌中MVD与Ki67的表达可能在大肠癌的发生、发展中发挥重要作用.%Objective. To investigate the microvessel density(MVD) and expression and clinical significance of Ki67 in colorectal adenocarcinoma. Methods The MVD and expression of Ki67 were determined by tissue microarray and immunohistochemical staining in 115 cases of colorectal adenocarcinoma(group A), 19 cases of colorectal adenoma(group B) and 12 cases of normal mucosa (group C). The relationship of Ki67 expression and MVD with clinicopathologic parameters was analyzed. Results There was no significant difference in the count of MVD marked by CD31 and CD34. The values of MVD in group A marked by CD31 and CD34 were 28. 30±13. 90 and 28. 73 ± 18. 90,respectively,which were higher than 8. 17±3. 93 and 10. 42±7. 32 in group C. MVD in group A was closely correlated to lymph node metastasis and the Dukes stage (P＜ 0. 05). The positive expression of Ki67 in group C was 16. 67％, which was significantly lower than 31.58％ in group B and 52. 17％ in group A. The expression of Ki67 was closely correlated to Dukes stage of colorectal adenocarcinoma(P＜0. 01). The MVD was positively correlated with the Ki67 expression. Conclusion MVD and Ki67 may play an important role in the carcinogenesis and development of colorectal carcinoma.