Objective To investigate the effect of granulocyte macrophage colony-stimulating factor(GM-CSF) on the hyperoxia-exposed lung injury in neonatal rats. Methods Thirty-two rats were equally randomized into four groups of A (exposed with 95% oxygen plus GM-CSF 9 Mg/kg intranasally injection) ,B(intranasally injected with GM-CSF 9 μg/kg),C(exposed with 95% oxygen plus normal saline intranasally injection) and D(normal saline intranasally injection). Seven days later, the wet/dry ratio(W/D) of lung tissues was calculated,and the number of leucocytes and the levels of monocyte chemoattractant protein-l(MCP-l) and interleukin-8 (IL-8) in the bronchoalveolar lavage fluid(BALF) were detected. The expression of MCP-1 mRNA in the lung tissue and the pathological score of lung injury were measured by RT-PCR and HE staining, respectively. Results Compared with groups of C and D,the pathological scores of lung injury, the W/D,the numbers of leucocytes and neutrophils in the BALF,the levels of MCP-1 and IL-8 and the expression of MCP-1 mRNA were all obviously increased in groups of A and B(P<0. 05). Except MCP-1 level and its mRNA expression, the indexes above were significantly different between group A and group B(P<0. 05). Conclusion GM-CSF can protect the the lungs from hyperoxia-induced injury in neonatal rats, which may be related to the inhibition of inflammatory reaction.%目的 探讨粒细胞-巨噬细胞集落刺激因子(GM-CSF)对高氧引起的新生大鼠肺损伤的干预作用.方法 32只大鼠随机均分为四组:95％氧浓度+GM-CSF 9 μg/kg(A)组、GM-CSF9 μg/kg(B)组、95％氧浓度+生理盐水(C)组和生理盐水(D)组.7d后计算肺湿质量与干质量比(W/D),检测支气管肺泡灌洗液(BALF)中白细胞数量、单核细胞趋化蛋白1(MCP-1)及白细胞介素8(IL-8)水平,RT-PCR检测肺组织中MCP-1 mRNA表达,HE染色进行肺损伤病理评分.结果 与C、D组相比,A、B组肺损伤评分及W/D、BALF中白细胞和中性粒细胞计数、MCP-1及IL-8水平、MCP-1 mRNA表达均明显增加(P＜0.05)；除MCP-1水平及其mRNA表达外,A组上述各指标与B组比较差异有统计学意义(P＜0.05).结论 GM-CSF对高氧暴露引起的新生大鼠肺损伤具有保护作用,这可能与损伤部位炎症反应受抑制有关.
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